Document of bibliographic reference 324148

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Comparative analysis of the soluble organic matrix of axial skeleton and sclerites of Corallium rubrum: insights for biomineralization

Abstract

We analysed the soluble organic matrix (SOM) of two biominerals formed by the same organism but differing by their morphological characteristics: the axial skeleton and the sclerites of Corallium rubrum. The results of 1D SDS-PAGE electrophoresis show for the two biominerals that SOM proteins bands have similar apparent molecular weight but differ in quantity. Further analysis by 2D electrophoresis reveals each protein band as a line of spots with different isoelectric points. Our results suggest that each SOM protein band consists of a mix of proteins and/or one unique protein with post-translational modifications. By immunohistochemistry, we show that antibodies raised against the SOM of axial skeleton and sclerites label the SOM of the two biominerals but also label the insoluble organic matrix suggesting the presence of common epitopes between the two biominerals and the two organic fractions.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000289448600005

Bibliographic citation

Debreuil, J.; Tambutté, S.; Zoccola, D.; Segonds, N.; Techer, N.; Allemand, D.; Tambutte, E. (2011). Comparative analysis of the soluble organic matrix of axial skeleton and sclerites of Corallium rubrum: insights for biomineralization. Comp. Biochem. Physiol. (B Biochem. Mol. Biol.) 159(1): 40-48. https://dx.doi.org/10.1016/j.cbpb.2011.01.007

Topic

Marine

Is peer reviewed

true