{"refrec":{"BRefID":328279,"RR":"<b>Hestetun, J.T.; Bye-Ingebrigtsen, E.; Nilsson, R.H.; Glover, A.G.; Johansen, P.-O.; Dahlgren, T.G.</b> (2020). Significant taxon sampling gaps in DNA databases limit the operational use of marine macrofauna metabarcoding. <i>Mar. Biodiv. 50(5)</i>: 70. <a href=\"https://dx.doi.org/10.1007/s12526-020-01093-5\" target=\"_blank\">https://dx.doi.org/10.1007/s12526-020-01093-5</a>","BEntID":321756,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biodiv. 50(5)</i>: 70. <a href=\"https://dx.doi.org/10.1007/s12526-020-01093-5\" target=\"_blank\">https://dx.doi.org/10.1007/s12526-020-01093-5</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hestetun, J.T.; Bye-Ingebrigtsen, E.; Nilsson, R.H.; Glover, A.G.; Johansen, P.-O.; Dahlgren, T.G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hestetun, J.T. <i>et al.</i>","Englishabstract":"Significant effort is spent on monitoring of benthic ecosystems through government funding or indirectly as a cost of business, and metabarcoding of environmental DNA samples has been suggested as a possible complement or alternative to current morphological methods to assess biodiversity. In metabarcoding, a public sequence database is typically used to match barcodes to species identity, but these databases are naturally incomplete. The North Sea oil and gas industry conducts large-scale environmental monitoring programs in one of the most heavily sampled marine areas worldwide and could therefore be considered a “best-case scenario” for macrofaunal metabarcoding. As a test case, we investigated the database coverage of two common metabarcoding markers, mitochondrial COI and the ribosomal rRNA 18S gene, for a complete list of 1802 macrofauna taxa reported from the North Sea monitoring region IV. For COI, species level barcode coverage was 50.4% in GenBank and 42.4% for public sequences in BOLD. For 18S, species level coverage was 36.4% in GenBank and 27.1% in SILVA. To see whether rare species were underrepresented, we investigated the most commonly reported species as a separate dataset but found only minor coverage increases. We conclude that compared to global figures, barcode coverage is high for this area, but that a significant effort remains to fill barcode databases to levels that would make metabarcoding operational as a taxonomic tool, including for the most common macrofaunal taxa.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Significant taxon sampling gaps in DNA databases limit the operational use of marine macrofauna metabarcoding","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:32:55.926290","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-08-26","DateCreate":"2020-08-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000560432400001","VABBcode":null,"OpenAcc":1,"DOI":"10.1007/s12526-020-01093-5"},"refs":null,"anarec":{"AnaID":328279,"PubliDate":2020,"Pagination":"70","XtraPublOfAnaID":null,"ISBN":null,"Volume":"50","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":141757,"SerRR":"Marine Biodiversity. 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