{"refrec":{"BRefID":328391,"RR":"<b>Randall, C.J.; Speaks, J.E.; Lager, C.; Hagedorn, M.; Llewellyn, L.; Pulak, R.; Thompson, J.; Bay, L.K.; Mead, D.; Heyward, A.J.; Negri, A.P.</b> (2020). Rapid counting and spectral sorting of live coral larvae using large-particle flow cytometry. <i>NPG Scientific Reports 10(1)</i>: 11 pp. <a href=\"https://dx.doi.org/10.1038/s41598-020-69491-0\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-020-69491-0</a>","BEntID":321868,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 10(1)</i>: 11 pp. <a href=\"https://dx.doi.org/10.1038/s41598-020-69491-0\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-020-69491-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Randall, C.J.; Speaks, J.E.; Lager, C.; Hagedorn, M.; Llewellyn, L.; Pulak, R.; Thompson, J.; Bay, L.K.; Mead, D.; Heyward, A.J.; Negri, A.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Randall, C.J. <i>et al.</i>","Englishabstract":"Research with coral embryos and larvae often requires laborious manual counting and sorting of individual specimens, usually via microscopy. Because many coral species spawn only once per year during a narrow temporal window, sample processing is a time-limiting step for research on the early life-history stages of corals. Flow cytometry, an automated technique for measuring and sorting particles, cells, and cell-clusters, is a potential solution to this bottleneck. Yet most flow cytometers do not accommodate live organisms of the size of most coral embryos (&gt; 250&nbsp;µm), and sample processing is often destructive. Here we tested the ability of a large-particle flow cytometer with a gentle pneumatic sorting mechanism to process and spectrally sort live and preserved <i>Montipora capitata</i> coral embryos and larvae. Average survival rates of mechanically-sorted larvae were over 90% and were comparable to those achieved by careful hand-sorting. Preserved eggs and embryos remained intact throughout the sorting process and were successfully sorted based on real-time size and fluorescence detection. In-line bright-field microscopy images were captured for each sample object as it passed through the flow-cell, enabling the identification of early-stage embryos (2-cell to morula stage). Samples were counted and sorted at an average rate of 4&nbsp;s larva<sup>−1</sup> and as high as 0.2&nbsp;s larva<sup>−1</sup> for high-density samples. Results presented here suggest that large-particle flow cytometry has the potential to significantly increase efficiency and accuracy of data collection and sample processing during time-limited coral spawning events, facilitating larger-scale and higher-replication studies with an expanded number of species.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Rapid counting and spectral sorting of live coral larvae using large-particle flow cytometry","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:31:44.558792","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-08-31","DateCreate":"2020-08-31","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000556411200018","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-020-69491-0"},"refs":null,"anarec":{"AnaID":328391,"PubliDate":2020,"Pagination":"11 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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