{"refrec":{"BRefID":328416,"RR":"<b>Alloul, A.; Wille, M.; Lucenti, P.; Bossier, P.; Van Stappen, G.; Vlaeminck, S.E.</b> (2021). Purple bacteria as added-value protein ingredient in shrimp feed: <i>Penaeus vannamei</i> growth performance, and tolerance against <i>Vibrio</i> and ammonia stress. <i>Aquaculture 530</i>: 735788. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2020.735788\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2020.735788</a>","BEntID":321893,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquaculture 530</i>: 735788. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2020.735788\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2020.735788</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Alloul, A.; Wille, M.; Lucenti, P.; Bossier, P.; Van Stappen, G.; Vlaeminck, S.E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Alloul, A. <i>et al.</i>","Englishabstract":"Aquafeeds contain protein ingredients such as fishmeal and soybean meal, yet their production puts pressure on the environment. Finding novel protein sources such as dried microbial biomass produced on recovered or renewable resources, so-called single-cell protein or microbial protein, can contribute to a more sustainable aquaculture industry. New microbial protein sources are emerging with photoheterotrophic grown purple non‑sulfur bacteria (PNSB) showing high potential, yet research of PNSB as added-value protein ingredient is limited. This research studied their use as a protein source for the white leg shrimp (<em>Penaeus vannamei</em>) and investigated the shrimp's tolerance against <em>Vibrio</em> and ammonia stress. A 28-day shrimp feeding trial was performed with a commercial formulation without PNSB as experimental control (diet i), two pure PNSB species, namely <em>Rhodopseudomonas palustris</em> (diets ii-iii)<em>, Rhodobacter capsulatus</em> (diets iv-v) at two protein inclusion levels of 5 and 11&nbsp;g PNSB<sub>protein</sub> 100&nbsp;g<sup>−1</sup> feed<sub>protein</sub> and a PNSB enriched culture at a protein inclusion level of 11&nbsp;g PNSB<sub>protein</sub> 100&nbsp;g<sup>−1</sup> feed<sub>protein</sub> (diet vi). For the shrimp fed with <em>Rb. capsulatus</em>, 5–25% higher individual weights (<em>p</em>&nbsp;&lt;&nbsp;.05) and better feed conversion ratios were observed relative to the commercial diet (1.3–1.4 vs. control 1.7&nbsp;g feed g<sup>−1</sup> biomass; <em>p</em>&nbsp;&lt;&nbsp;.05). The diet containing <em>Rps. palustris</em> at 5&nbsp;g PNSB<sub>protein</sub> 100&nbsp;g<sup>−1</sup> feed<sub>protein</sub> inclusion also showed higher individual weights (26%, <em>p</em>&nbsp;&lt;&nbsp;.05) and a better feed conversion ratio compared to the commercial feed (1.3 vs. control 1.7&nbsp;g feed g<sup>−1</sup> biomass; <em>p</em>&nbsp;&lt;&nbsp;.05). The challenge test subsequent to the feeding trial showed a higher tolerance against ammonia (3&nbsp;mg&nbsp;N&nbsp;L<sup>−1</sup>) for shrimp fed with <em>Rps. palustris</em> (survival 63–75% vs. 8% commercial diet; <em>p</em>&nbsp;&lt;&nbsp;.05). For a post-feeding challenge test with <em>Vibrio parahaemolyticus</em> TW01, mortality rates were equal among all treatments. Yet, <em>in vitro</em> tests in 96-Well plates and agar spot assays showed that the PNSB species (i) <em>Rps. palustris,</em> (ii) <em>Rb. capsulatus,</em> (iii) <em>Rb. sphaeroides,</em> (iv) <em>Rhodospirillum rubrum</em> and (v) <em>Afifella marina</em> suppressed the pathogens <em>V. parahaemolyticus</em> TW01 and <em>V. campbellii</em> LMG 21363. Overall, this study demonstrated the potential of PNSB as an added-value protein ingredient in shrimp nursery feed. This can contribute to a circular economy, as PNSB can be cultivated on recovered or renewable resources (e.g. wastewater).","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Purple bacteria as added-value protein ingredient in shrimp feed: <i>Penaeus vannamei</i> growth performance, and tolerance against <i>Vibrio</i> and ammonia stress","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Acute hepatopancreatic necrosis disease; Early mortality syndrome; Purple phototrophic bacteria; Alternative protein source; Raceway pond","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-05-17","DateCreate":"2020-09-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000582169700073","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquaculture.2020.735788"},"refs":null,"anarec":{"AnaID":328416,"PubliDate":2021,"Pagination":"735788","XtraPublOfAnaID":null,"ISBN":null,"Volume":"530","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. 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ISSN 0044-8486; e-ISSN 1873-5622","StandardTitleSer":"Aquaculture","ISSN":"0044-8486","AbbrevSer":"Aquaculture","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Alloul","Firstname":"Abbas","Initials":"A.","Affiliation":"Research Group of Sustainable Energy, Air and Water Technology, Department of Bioscience Engineering, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":321893,"AutID":423290,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"DuEL","InsFSN":"Universiteit Antwerpen; Faculteit Wetenschappen; Departement Bio-ingenieurswetenschappen; Duurzame Energie- en Lucht- en Watertechnologie","ORCID":"0000-0003-4125-7470","PersID":39390,"InsID":14390},{"AutName":"Wille","Firstname":"Mathieu","Initials":"M.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":321893,"AutID":145122,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; 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