{"refrec":{"BRefID":329344,"RR":"<b>Leide, J.; Nierop, K.G.J.; Deininger, A.-C.; Staiger, S.; Riederer, M.; de Leeuw, J.W.</b> (2020). Leaf cuticle analyses: implications for the existence of cutan/non-ester cutin and its biosynthetic origin. <i>Ann. Bot. 126(1)</i>: 141-162. <a href=\"https://dx.doi.org/10.1093/aob/mcaa056\" target=\"_blank\">https://dx.doi.org/10.1093/aob/mcaa056</a>","BEntID":322950,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Ann. Bot. 126(1)</i>: 141-162. <a href=\"https://dx.doi.org/10.1093/aob/mcaa056\" target=\"_blank\">https://dx.doi.org/10.1093/aob/mcaa056</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Leide, J.; Nierop, K.G.J.; Deininger, A.-C.; Staiger, S.; Riederer, M.; de Leeuw, J.W.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Leide, J. <i>et al.</i>","Englishabstract":"<p>    <strong>Background and Aims</strong></p><p>    The cuticle of a limited number of plant species contains cutan, a    chemically highly resistant biopolymer. As yet, the biosynthesis of cutan    is not fully understood. Attempting to further unravel the origin of cutan,    we analysed the chemical composition of enzymatically isolated cuticular    membranes of <em>Agave americana</em> leaves.</p><p>    <strong>Methods</strong></p><p>    Cuticular waxes were extracted with organic solvents. Subsequently, the    dewaxed cuticular membrane was depolymerized by acid-catalysed    transesterification yielding cutin monomers and cutan, a non-hydrolysable,    cuticular membrane residue. The cutan matrix was analysed by thermal    extraction, flash pyrolysis and thermally assisted hydrolysis and    methylation to elucidate the monomeric composition and deduce a putative    biosynthetic origin.</p><p>    <strong>Key Results</strong></p><p>    According to gas chromatography–mass spectrometry analyses, the cuticular    waxes of <em>A. americana</em> contained primarily very-long-chain alkanoic    acids and primary alkanols dominated by C<sub>32</sub>, whereas the cutin    biopolyester of <em>A. americana</em> mainly consisted of 9,10-epoxy    ω-hydroxy and 9,10,ω-trihydroxy C<sub>18</sub> alkanoic acids. The main    aliphatic cutan monomers were alkanoic acids, primary alkanols, ω-hydroxyalkanoic acids and alkane-α,ω-diols ranging predominantly from C    <sub>28</sub> to C<sub>34</sub> and maximizing at C<sub>32</sub>. Minor    contributions of benzene-1,3,5-triol and derivatives suggested that these    aromatic moieties form the polymeric core of cutan, to which the aliphatic    moieties are linked via ester and possibly ether bonds.</p><p>    <strong>Conclusions</strong></p><p>    High similarity of aliphatic moieties in the cutan and the cuticular wax    component indicated a common biosynthetic origin. In order to exclude    species-specific peculiarities of <em>A. americana</em> and to place ourresults in a broader context, cuticular waxes, cutin and cutan of<em>Clivia miniata</em>, <em>Ficus elastica</em> and    <em>Prunus laurocerasus</em> leaves were also investigated. A detailed    comparison showed compositional and structural differences, indicated that    cutan was only found in leaves of perennial evergreen <em>A. americana</em>    and <em>C. miniata</em>, and made clear that the phenomenon of cutan is    possibly less present in plant species than suggested in the literature.</p>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Leaf cuticle analyses: implications for the existence of cutan/non-ester cutin and its biosynthetic origin","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:32:34.025042","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<i>Agave americana</i>, <i>Clivia miniata</i>, <i>Ficus elastica</i>; <i>Prunus laurocerasus</i>; cuticular waxes; cutin; cutan; non-ester cutin; Fourier transform infrared spectroscopy (FTIR); flash pyrolysis; thermally assisted hydrolysis and methylation (THM)","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2021-01-14","DateCreate":"2020-09-15","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000561731100010","VABBcode":null,"OpenAcc":0,"DOI":"10.1093/aob/mcaa056"},"refs":null,"anarec":{"AnaID":329344,"PubliDate":2020,"Pagination":"141-162","XtraPublOfAnaID":null,"ISBN":null,"Volume":"126","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42136,"SerRR":"Annals of Botany. 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