{"refrec":{"BRefID":329410,"RR":"<b>Bozzo, M.; Candiani, S.; Schubert, M.</b> (2020). Whole mount <i>in situ</i> hybridization and immunohistochemistry for studying retinoic acid signaling in developing amphioxus. <i>Methods Enzymol. 637</i>: 419-452. <a href=\"https://dx.doi.org/10.1016/bs.mie.2020.03.007\" target=\"_blank\">https://dx.doi.org/10.1016/bs.mie.2020.03.007</a>","BEntID":323016,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Methods Enzymol. 637</i>: 419-452. <a href=\"https://dx.doi.org/10.1016/bs.mie.2020.03.007\" target=\"_blank\">https://dx.doi.org/10.1016/bs.mie.2020.03.007</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bozzo, M.; Candiani, S.; Schubert, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bozzo, M.; Candiani, S.; Schubert, M.","Englishabstract":"Retinoic acid (RA) is a vitamin A-derived signaling molecule acting during development and in the adult. This chapter provides protocols to characterize the role of RA signaling during development of the invertebrate chordate amphioxus. As sister group to all other chordates and characterized by the most vertebrate-like RA signaling system of all invertebrates, amphioxus is an important model for studying the evolution of RA signaling. Focusing on the development of GABAergic neurons in the amphioxus central nervous system, we provide detailed protocols for maintaining and breeding adult animals, for performing pharmacological treatments of embryos and for analyzing the effects of these treatments by whole mount <em>in situ</em> hybridization and immunohistochemistry coupled to confocal microscopy.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Whole mount <i>in situ</i> hybridization and immunohistochemistry for studying retinoic acid signaling in developing amphioxus","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-05 01:31:21.347183","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Amphioxus; Cephalochordate; Retinoic acid signaling; Pharmacological treatments; Immunohistochemistry; Whole mount in situ hybridization; Fluorescent and confocal imaging; Neurobiology; GABA; GAD","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-09-17","DateCreate":"2020-09-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000556296000017","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/bs.mie.2020.03.007"},"refs":null,"anarec":{"AnaID":329410,"PubliDate":2020,"Pagination":"419-452","XtraPublOfAnaID":null,"ISBN":null,"Volume":"637","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43397,"SerRR":"Methods in enzymology. 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