{"refrec":{"BRefID":329412,"RR":"<b>Li, Z.; Wang, Y.; Vasylieva, N.; Wan, D.; Yin, Z.; Dong, J.; Hammock, B.D.</b> (2020). An ultrasensitive bioluminescent enzyme immunoassay based on nanobody/nanoluciferase heptamer fusion for the detection of Tetrabromobisphenol A in sediment. <i>Anal. Chem. 92(14)</i>: 10083-10090. <a href=\"https://dx.doi.org/10.1021/acs.analchem.0c01908\" target=\"_blank\">https://dx.doi.org/10.1021/acs.analchem.0c01908</a>","BEntID":323018,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Anal. Chem. 92(14)</i>: 10083-10090. <a href=\"https://dx.doi.org/10.1021/acs.analchem.0c01908\" target=\"_blank\">https://dx.doi.org/10.1021/acs.analchem.0c01908</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Li, Z.; Wang, Y.; Vasylieva, N.; Wan, D.; Yin, Z.; Dong, J.; Hammock, B.D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Li, Z. <i>et al.</i>","Englishabstract":"Tetrabromobisphenol A (TBBPA) is a flame retardant and has become a widely concerning environmental pollutant. An ultrasensitive nanobody-based immunoassay was developed to monitor the exposure of TBBPA in sediment. First, the anti-TBBPA nanobody was fused with nanoluciferase, and then a one-step bioluminescent enzyme immunoassay (BLEIA) was developed with high sensitivity for TBBPA, with a maximum half inhibition concentration (IC<sub>50</sub>) at 187 pg/mL. Although approximately 10-fold higher sensitivity can be achieved by this developed BLEIA than by the classical two-step ELISA (IC<sub>50</sub> at 1778 pg/mL), it is still a challenge to detect trace TBBPA in sediment samples reliably due to the relatively high matrix effect. To further improve the performance of this one-step BLEIA, a C4b-binding protein (C4BP) was inserted as a self-assembling linker between the nanobody and nanoluciferase. Therefore, a heptamer fusion containing seven binders and seven tracers was generated. This reagent improved the binding capacity and signal amplification. The one-step heptamer plus BLEIA based on this immune-reagent shows an additional 7-fold improvement of sensitivity, with the IC<sub>50</sub> of 28.9 pg/mL and the limit of detection as low as 2.5 pg/mL. The proposed assay was further applied to determine the trace TBBPA in sediment, and the recovery was within 92–103%. Taking advantage of this heptamer fusion, one-step BLEIA can serve as a powerful tool for fast detection of trace TBBPA in the sediment samples.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"An ultrasensitive bioluminescent enzyme immunoassay based on nanobody/nanoluciferase heptamer fusion for the detection of Tetrabromobisphenol A in sediment","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-09 01:32:08.738337","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-09-17","DateCreate":"2020-09-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000554986200080","VABBcode":null,"OpenAcc":0,"DOI":"10.1021/acs.analchem.0c01908"},"refs":null,"anarec":{"AnaID":329412,"PubliDate":2020,"Pagination":"10083-10090","XtraPublOfAnaID":null,"ISBN":null,"Volume":"92","Issue":"14","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42100,"SerRR":"Analytical chemistry. 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