{"refrec":{"BRefID":329466,"RR":"Tsirigoti, A.; Kupper, C; Gachon, C.M.M.; Katsaros, C. (2014). Cytoskeleton organisation during the infection of three brown algal species Ectocarpus siliculosus, Ectocarpus crouaniorum and Pylaiella littoralis, by the intracellular marine oomycete Eurychasma dicksonii. Plant Biol. 16(1): 272-281. https://dx.doi.org/10.1111/plb.12041","BEntID":323072,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". Plant Biol. 16(1): 272-281. https://dx.doi.org/10.1111/plb.12041","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tsirigoti, A.; Kupper, C; Gachon, C.M.M.; Katsaros, C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tsirigoti, A. et al.","Englishabstract":"Oomycete diseases in seaweeds are probably widespread and of significant ecological and economic impact, but overall still poorly understood. This study investigates the organisation of the cytoskeleton during infection of three brown algal species, Pylaiella littoralis, Ectocarpus siliculosus, and Ectocarpus crouaniorum, by the basal marine oomycete Eurychasma dicksonii. Immunofluorescence staining of tubulin revealed how the development of this intracellular biotrophic pathogen impacts on microtubule (MT) organisation of its algal host. The host MT cytoskeleton remains normal and organised by the centrosome until very late stages of the infection. Additionally, the organisation of the parasite's cytoskeleton was examined. During mitosis of the E. dicksonii nucleus the MT focal point (microtubule organisation centre, MTOC, putative centrosome) duplicates and each daughter MTOC migrates to opposite poles of the nucleus. This similarity in MT organisation between the host and pathogen reflects the relatively close phylogenetic relationship between oomycetes and brown algae. Moreover, actin labelling with rhodamineâphalloidin in E. dicksonii revealed typical images of actin dots connected by fine actin filament bundles in the cortical cytoplasm. The functional and phylogenetic implications of our observations are discussed.","AbstractOtherLang":"Actin; brown algae; cytoskeleton; host;infection; microtubule; oomycete.","BibLvlCode":"AS","StandardTitle":"Cytoskeleton organisation during the infection of three brown algal species Ectocarpus siliculosus, Ectocarpus crouaniorum and Pylaiella littoralis, by the intracellular marine oomycete Eurychasma dicksonii","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-09 01:32:08.753961","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Actin; brown algae; cytoskeleton; host; infection; microtubule; oomycete","OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2020-09-25","DateCreate":"2020-09-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000328826900032","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/plb.12041"},"refs":null,"anarec":{"AnaID":329466,"PubliDate":2014,"Pagination":"272-281","XtraPublOfAnaID":null,"ISBN":null,"Volume":"16","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":183071,"SerRR":"Plant Biology. Blackwell Publishing: New York; Stuttgart. ISSN 1435-8603; e-ISSN 1438-8677","StandardTitleSer":"Plant Biology","ISSN":"1435-8603","AbbrevSer":"Plant Biol.","StandardTitleMon":null,"StartPage":272,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Tsirigoti","Firstname":"Amerssa","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":323072,"AutID":271005,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kupper","Firstname":"F","Initials":"C","Affiliation":"Univ Aberdeen, Oceanlab, Newburgh AB41 6AA, Aberdeen, Scotland.","Discriminator":null,"CorporateFlag":0,"BEntID":323072,"AutID":194915,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Gachon","Firstname":null,"Initials":"C.M.M.","Affiliation":"Scottish Assoc Marine Sci Culture Collect Algae &, Scottish Marine Inst,Oban PA37 1QA, Argyll, Scotland.","Discriminator":null,"CorporateFlag":0,"BEntID":323072,"AutID":181424,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Katsaros","Firstname":"Christos","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":323072,"AutID":271006,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":351303,"LibID":36,"BRefID":329466,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"351303","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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