{"refrec":{"BRefID":329605,"RR":"<b>Kher, C.P.; Doerder, F.P.; Cooper, J.; Ikonomi, P.; Achilles-Day, U.; Küpper, F.C.; Lynn, D.H.</b> (2011). Barcoding <i>Tetrahymena</i>: Discriminating species and identifying unknowns using the cytochrome c oxidase Subunit I (cox-1) barcode. <i>Protist 162(1)</i>: 2-13. <a href=\"https://dx.doi.org/10.1016/j.protis.2010.03.004\" target=\"_blank\">https://dx.doi.org/10.1016/j.protis.2010.03.004</a>","BEntID":323212,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Protist 162(1)</i>: 2-13. <a href=\"https://dx.doi.org/10.1016/j.protis.2010.03.004\" target=\"_blank\">https://dx.doi.org/10.1016/j.protis.2010.03.004</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kher, C.P.; Doerder, F.P.; Cooper, J.; Ikonomi, P.; Achilles-Day, U.; Küpper, F.C.; Lynn, D.H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kher, C.P. <i>et al.</i>","Englishabstract":"DNA barcoding using the mitochondrial cytochrome</strong> <em><strong>c</strong></em> <strong>oxidase subunit I (cox-1) gene has recently gained popularity as a tool for species identification of a variety of taxa. The primary objective of our research was to explore the efficacy of using cox-1 barcoding for species identification within the genus</strong> <em><strong>Tetrahymena</strong></em><strong>. We first increased intraspecific sampling for</strong> <em><strong>Tetrahymena canadensis, Tetrahymena hegewischi, Tetrahymena pyriformis, Tetrahymena rostrata, Tetrahymena thermophila, and Tetrahymena tropicalis</strong></em><strong>. Increased sampling efforts show that intraspecific sequence divergence is typically less than 1%, though it may be more in some species. The barcoding also showed that some strains might be misidentified or mislabeled. We also used cox-1 barcodes to provide species identifications for 51 unidentified environmental isolates, with a success rate of 98%. 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