{"refrec":{"BRefID":330294,"RR":"<b>van Winden, J.F.; Talbot, H.M.; Reichart, G.-J.; McNamara, N.P.; Benthien, A.; Sinninghe Damsté, J.S.</b> (2020). Influence of temperature on the δ<sup>13</sup>C values and distribution of methanotroph‐related hopanoids in <i>Sphagnum</i>‐dominated peat bogs. <i>Geobiol. 18(4)</i>: 497-507. <a href=\"https://dx.doi.org/10.1111/gbi.12389\" target=\"_blank\">https://dx.doi.org/10.1111/gbi.12389</a>","BEntID":323904,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Geobiol. 18(4)</i>: 497-507. <a href=\"https://dx.doi.org/10.1111/gbi.12389\" target=\"_blank\">https://dx.doi.org/10.1111/gbi.12389</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"van Winden, J.F.; Talbot, H.M.; Reichart, G.-J.; McNamara, N.P.; Benthien, A.; Sinninghe Damsté, J.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"van Winden, J.F. <i>et al.</i>","Englishabstract":"Methane emissions from peat bogs are mitigated by methanotrophs, which live in symbiosis with peat moss (e.g. <i>Sphagnum</i>). Here, we investigate the influence of temperature and resultant changes in methane fluxes on <i>Sphagnum</i> and methanotroph‐related biomarkers, evaluating their potential as proxies in ancient bogs. A pulse‐chase experiment using <sup>13</sup>C‐labelled methane in the field clearly showed label uptake in diploptene, a biomarker for methanotrophs, demonstrating in situ methanotrophic activity in <i>Sphagnum</i> under natural conditions. Peat cores containing live <i>Sphagnum</i> were incubated at 5, 10, 15, 20 and 25°C for two months, causing differences in net methane fluxes. The natural δ<sup>13</sup>C values of diploptene extracted from <i>Sphagnum</i> showed a strong correlation with temperature and methane production. The δ<sup>13</sup>C values ranged from −34‰ at 5°C to −41‰ at 25°C. These results are best explained by enhanced expression of the methanotrophic enzymatic isotope effect at higher methane concentrations. Hence, δ<sup>13</sup>C values of diploptene, or its diagenetic products, potentially provide a useful tool to assess methanotrophic activity in past environments. Increased methane fluxes towards <i>Sphagnum</i> did not affect δ<sup>13</sup>C values of bulk <i>Sphagnum</i> and its specific marker, the C<sub>23</sub> <i>n</i>‐alkane. The concentration of methanotroph‐specific bacteriohopanepolyols (BHPs), aminobacteriohopanetetrol (aminotetrol, characteristic for type II and to a lesser extent type I methanotrophs) and aminobacteriohopanepentol (aminopentol, a marker for type I methanotrophs) showed a non‐linear response to increased methane fluxes, with relatively high abundances at 25°C compared to those at 20°C or below. Aminotetrol was more abundant than aminopentol, in contrast to similar abundances of aminotetrol and aminopentol in fresh <i>Sphagnum</i>. This probably indicates that type II methanotrophs became prevalent under the experimental conditions relative to type I methanotrophs. Even though BHP concentrations may not directly reflect bacterial activity, they may provide insight into the presence of different types of methanotrophs.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Influence of temperature on the δ<sup>13</sup>C values and distribution of methanotroph‐related hopanoids in <i>Sphagnum</i>‐dominated peat bogs","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:32:19.787079","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"bacteriohopanepolyol; carbon; isotopes; methane; methanotroph; <i>Sphagnum</i>; temperature","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-10-20","DateCreate":"2020-10-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000563550400001","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/gbi.12389"},"refs":null,"anarec":{"AnaID":330294,"PubliDate":2020,"Pagination":"497-507","XtraPublOfAnaID":null,"ISBN":null,"Volume":"18","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":209856,"SerRR":"Geobiology. 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