{"refrec":{"BRefID":330476,"RR":"<b>Davidov, K.; Iankelevich-Kounio, E.; Yakovenko, I.; Koucherov, Y.; Rubin-Blum, M.; Oren, M.</b> (2020). Identification of plastic-associated species in the Mediterranean Sea using DNA metabarcoding with Nanopore MinION. <i>NPG Scientific Reports 10(1)</i>: 11 pp. <a href=\"https://dx.doi.org/10.1038/s41598-020-74180-z\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-020-74180-z</a>","BEntID":324086,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 10(1)</i>: 11 pp. <a href=\"https://dx.doi.org/10.1038/s41598-020-74180-z\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-020-74180-z</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Davidov, K.; Iankelevich-Kounio, E.; Yakovenko, I.; Koucherov, Y.; Rubin-Blum, M.; Oren, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Davidov, K. <i>et al.</i>","Englishabstract":"Plastic debris in the ocean form a new ecosystem, termed ‘plastisphere’, which hosts a variety of marine organisms. Recent studies implemented DNA metabarcoding to characterize the taxonomic composition of the plastisphere in different areas of the world. In this study, we used a modified metabarcoding approach which was based on longer barcode sequences for the characterization of the plastisphere biota. We compared the microbiome of polyethylene food bags after 1 month at sea to the free-living biome in two proximal but environmentally different locations on the Mediterranean coast of Israel. We targeted the full 1.5 kb-long 16S rRNA gene for bacteria and 0.4–0.8 kb-long regions within the 18S rRNA, ITS, tufA and COI loci for eukaryotes. The taxonomic barcodes were sequenced using Oxford Nanopore Technology with multiplexing on a single MinION flow cell. We identified between 1249 and 2141 species in each of the plastic samples, of which 61 species (34 bacteria and 27 eukaryotes) were categorized as plastic-specific, including species that belong to known hydrocarbon-degrading genera. In addition to a large prokaryotes repertoire, our results, supported by scanning electron microscopy, depict a surprisingly high biodiversity of eukaryotes within the plastisphere with a dominant presence of diatoms as well as other protists, algae and fungi.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Identification of plastic-associated species in the Mediterranean Sea using DNA metabarcoding with Nanopore MinION","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-09 01:32:09.394644","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-10-23","DateCreate":"2020-10-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000585841900014","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-020-74180-z"},"refs":null,"anarec":{"AnaID":330476,"PubliDate":2020,"Pagination":"11 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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