{"refrec":{"BRefID":330979,"RR":"<b>González-Ramos, J.; Agell, G.; Uriz, M.J.</b> (2015). Microsatellites from sponge genomes: the number necessary for detecting genetic structure in <i>Hemimycale columella</i> populations. <i>Aquat. Biol. 24(1)</i>: 25-34. <a href=\"https://dx.doi.org/10.3354/ab00630\" target=\"_blank\">https://dx.doi.org/10.3354/ab00630</a>","BEntID":324590,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Biol. 24(1)</i>: 25-34. <a href=\"https://dx.doi.org/10.3354/ab00630\" target=\"_blank\">https://dx.doi.org/10.3354/ab00630</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"González-Ramos, J.; Agell, G.; Uriz, M.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"González-Ramos, J.; Agell, G.; Uriz, M.J.","Englishabstract":"Using next-generation sequencing technology, we designed a pool of microsatellite primers and amplified them in 2 physically isolated populations of the Atlanto-Mediterranean sponge <i>Hemimycale columella</i>, which exhibits particular evolutionary, biological, and ecological features. The species has contrasting life cycles in shallow and relatively deep waters, and releases small, predictably low-dispersal larvae. This study experimentally evaluated how many polymorphic microsatellites would be necessary to detect genetic structure in <i>H. columella</i> populations, and whether or not the use of compound loci is advisable. By sequencing 1/2 454 GS-FLX run, we obtained a total of 4208 sequences enclosing microsatellite motifs. We selected 20 microsatellites, 15 of which proved adequate for the genetic study of the sponge populations. The 2 analyzed populations were genetically structured with all microsatellite combinations assayed, and the values of the <i>D</i><sub>est</sub> and <i>F</i><sub>ST</sub> statistics did not increase with increasing number of loci. A weak signal of genetic structure, however, was shown in bar plots representing membership coefficients for each individual to each sampling location even with all loci. We conclude that a few polymorphic loci can detect structure in <i>H. columella</i> populations, but using 12 or more loci notably enhances the power of the analyses. The study also describes a low-cost protocol for obtaining microsatellites by the hundreds from non-model, ecologically relevant species, which can be used to provide information about population isolation, adaptation, and vulnerability.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Microsatellites from sponge genomes: the number necessary for detecting genetic structure in <i>Hemimycale columella</i> populations","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:32:08.407709","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2020-11-16","DateCreate":"2020-11-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000361045800004","VABBcode":null,"OpenAcc":1,"DOI":"10.3354/ab00630"},"refs":null,"anarec":{"AnaID":330979,"PubliDate":2015,"Pagination":"25-34","XtraPublOfAnaID":null,"ISBN":null,"Volume":"24","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123983,"SerRR":"Aquatic Biology. 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