Document of bibliographic reference 331002

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Quantitative comparison of bacterial communities in two Mediterranean sponges

Abstract

Marine sponges can host in their tissues abundant and diverse bacterial communities. Lack of truly quantitative data on bacterial abundance and dynamics limits our understanding of the organization and functioning of these endobiotic communities. In this technical note, we describe a quantitative polymerase chain reaction approach to quantify the relative abundance of multiple clades of three major sponge-associated bacterial phyla: Chloroflexi, Acidobacteria, and Actinobacteria. To test our approach we used the Mediterranean sponges Spongia lamella and Aplysina aerophoba. We designed five out of the six primer sets used in our study. We tested the new primer sets for specificity and optimized their conditions. Our preliminary data showed that Spongia lamella had larger bacterial abundance than Aplysina aerophoba, except for one clade of Chloroflexi. The two Chloroflexi clades investigated in our study amplified a fraction of the Chloroflexi present in Spongia lamella and most of what is present in Aplysina aerophoba, suggesting a more diverse Chloroflexi population in Spongia lamella than in Aplysina aerophoba. This quantitative technique has a great potential to provide a rapid and robust assessment of sponge microbial target and could contribute to deciphering the complexity of these largely unknown host-symbiont interactions.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000285308100004

Bibliographic citation

Noyer, C.; Hamilton, A.; Sacristan-Soriano, O.; Becerro, M.A. (2010). Quantitative comparison of bacterial communities in two Mediterranean sponges. Symbiosis 51(3): 239-243. https://dx.doi.org/10.1007/s13199-010-0082-2

Topic

Marine

Is peer reviewed

true