{"refrec":{"BRefID":331097,"RR":"<b>Peperzak, L.; van Bleijswijk, J.</b> (2021). False-positive enterococci counts in seawater with the IDEXX Enterolert-E most probable number technique caused by <i>Bacillus licheniformis</i>. <i>Environm. Sc. & Poll. Res. 28</i>: 10654-10660. <a href=\"https://doi.org/10.1007/s11356-020-11342-6\" target=\"_blank\">https://doi.org/10.1007/s11356-020-11342-6</a>","BEntID":324708,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Environm. Sc. & Poll. Res. 28</i>: 10654-10660. <a href=\"https://doi.org/10.1007/s11356-020-11342-6\" target=\"_blank\">https://doi.org/10.1007/s11356-020-11342-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Peperzak, L.; van Bleijswijk, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Peperzak, L.; van Bleijswijk, J.","Englishabstract":"Enterolert-E is an easy-to-use method for the enumeration of enterococci in water samples as an indicator of fecal pollution. This most probable number technique replaced the laborious and more time-consuming MEA-BEA plating method, and it is used extensively in ballast water testing and monitoring. In spring 2018, the Control Union Water ballast water test facility measured high enterococci concentrations in Wadden Sea water without any correlation with polluted freshwater input. By isolating bacteria from samples incubated in Enterolert-E culture medium, followed by analyses of colony morphology and DNA, it is shown that these erroneously high concentrations were caused by <i>Bacillus licheniformis</i>, a gram-positive rod-shaped chlorine-resistant bacterium. It is concluded that control analyses or the MEA-BEA method or dilution to reduce salinity must be performed when high enterococci concentrations are measured in water samples that are not suspected to be polluted.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"False-positive enterococci counts in seawater with the IDEXX Enterolert-E most probable number technique caused by <i>Bacillus licheniformis</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:32.853369","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Ballast water; Enterococci; False positive; IDEXX; Enterolert-E; <i>Bacillus licheniformis</i>; Wadden Sea","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-04-24","DateCreate":"2020-11-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000582312800005","VABBcode":null,"OpenAcc":1,"DOI":"10.1007/s11356-020-11342-6"},"refs":null,"anarec":{"AnaID":331097,"PubliDate":2021,"Pagination":"10654-10660","XtraPublOfAnaID":null,"ISBN":null,"Volume":"28","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45312,"SerRR":"Environmental Science and Pollution Research. Springer: Heidelberg; Berlin.  ISSN 0944-1344; e-ISSN 1614-7499","StandardTitleSer":"Environmental Science and Pollution Research","ISSN":"0944-1344","AbbrevSer":"Environm. Sc. & Poll. Res.","StandardTitleMon":null,"StartPage":10654,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Peperzak","Firstname":"Louis","Initials":"L.","Affiliation":"EDS","Discriminator":null,"CorporateFlag":0,"BEntID":324708,"AutID":213438,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"EDS","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Estuarine and Delta Systems","ORCID":"0000-0003-0691-2521","PersID":29210,"InsID":13657},{"AutName":"van Bleijswijk","Firstname":"Judith","Initials":"J.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":324708,"AutID":236648,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0002-4748-8168","PersID":11075,"InsID":13655}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":324708,"BEntPrivate":null,"availability":[{"BInstID":359409,"LibID":2779,"BRefID":331097,"EmbargoDate":"24/04/2021","FullEmbargoDate":{"date":"2021-04-24 00:00:00.000000","timezone_type":3,"timezone":"Europe/Brussels"},"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"359409","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":262,"Classification":"NIOZ Open Repository - Accepted Manuscripts","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null},{"BInstID":359397,"LibID":2779,"BRefID":331097,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"359397","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":261,"Classification":"NIOZ files","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":null,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Springer","City":"Heidelberg; Berlin"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Bacillus licheniformis"},{"TaxTerm":"Enterococcus"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":324708,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":324708,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://doi.org/10.1007/s11356-020-11342-6","externalID":"10.1007/s11356-020-11342-6","URLTypeCode":"DOI","URLID":88064,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Bacillus licheniformis","AphiaID":null,"TaxtID":36272},{"TaxTerm":"Enterococcus","AphiaID":565140,"TaxtID":40551}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Bacillus licheniformis","DutchTerm":null,"AphiaID":null,"TaxtID":36272},{"TaxTerm":"Enterococcus","DutchTerm":null,"AphiaID":565140,"TaxtID":40551}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Leonne.van.der.Weegen@nioz.nl","newSesDate":{"date":"2020-11-17 09:03:16.680000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"T'Jampens, Roeland, R.","updSesDate":{"date":"2021-04-24 01:40:42.610000","timezone_type":3,"timezone":"Europe/Brussels"}}}
