{"refrec":{"BRefID":331112,"RR":"<b>Pimentel-Elardo, S.; Wehrl, M.; Friedrich, A.B.; Jensen, P.R.; Hentschel, U.</b> (2003). Isolation of planctomycetes from <i>Aplysina</i> sponges. <i>Aquat. Microb. Ecol. 33</i>: 239-245. <a href=\"https://dx.doi.org/10.3354/ame033239\" target=\"_blank\">https://dx.doi.org/10.3354/ame033239</a>","BEntID":324723,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Microb. Ecol. 33</i>: 239-245. <a href=\"https://dx.doi.org/10.3354/ame033239\" target=\"_blank\">https://dx.doi.org/10.3354/ame033239</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Pimentel-Elardo, S.; Wehrl, M.; Friedrich, A.B.; Jensen, P.R.; Hentschel, U.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Pimentel-Elardo, S. <i>et al.</i>","Englishabstract":"There is mounting molecular evidence that bacteria belonging to the phylum Planctomycetes are abundant in marine sponges including members of the genus <i>Aplysina</i>. In an attempt to culture planctomycete bacteria from <i>Aplysina</i> sponges, 116 bacterial strains were isolated on selective oligotrophic media. Screening of the strain collection by fluorescence <i>in situ</i> hybridization with the planctomycete-specific probe Pla46 yielded 3 positive candidates. Nearly complete sequencing of the respective 16S rRNA genes revealed that the isolates were affiliated with 2 distinct clusters of the genus <i>Pirellula</i>: 1 isolate was obtained from a Mediterranean sponge, 1 from a Caribbean sponge and a third from Caribbean seawater. To our knowledge this is the first report of cultured Planctomycetes from marine sponges. The isolates grew slowly on oligotrophic media and failed to grow on nutrient-rich media. <i>Pirellula</i> sp. Strain 797 was pink-pigmented while the other 2 isolates, 16 and 81, were non-pigmented. Transmission electron microscopy revealed a pear- or droplet-shaped cell morphology that is characteristic of the genus <i>Pirellula</i>. The application of strain-specific oligonucleotide probes to sponge tissue cryosections showed that the isolates contribute only a minor fraction to the total microbial community that is associated with <i>Aplysina</i> spp. sponges.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Isolation of planctomycetes from <i>Aplysina</i> sponges","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-23 01:33:07.055354","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Pirellula · Planctomycete · Aplysina · Sponge · Porifera · Sponge-associated microorganism · 16S rDNA","OtherDescriptors":null,"Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2020-11-17","DateCreate":"2020-11-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000188418000004","VABBcode":null,"OpenAcc":1,"DOI":"10.3354/ame033239"},"refs":null,"anarec":{"AnaID":331112,"PubliDate":2003,"Pagination":"239-245","XtraPublOfAnaID":null,"ISBN":null,"Volume":"33","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42201,"SerRR":"Aquatic Microbial Ecology. 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