{"refrec":{"BRefID":331200,"RR":"<b>Ludevese-Pascual, G.; Laranja, J.L.; Ahmed, F.; Amar, E.; De Troch, M.; Bossier, P.; De Schryver, P.</b> (2020). Lipids and fatty acid composition in the crustacean model organism <i>Artemia</i> sp. as influenced by poly‐β‐hydroxybutyrate (PHB) supplementation. <i>Aquacult. Nutr. 26(6)</i>: 2235-2244. <a href=\"https://dx.doi.org/10.1111/anu.13160\" target=\"_blank\">https://dx.doi.org/10.1111/anu.13160</a>","BEntID":324811,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquacult. Nutr. 26(6)</i>: 2235-2244. <a href=\"https://dx.doi.org/10.1111/anu.13160\" target=\"_blank\">https://dx.doi.org/10.1111/anu.13160</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ludevese-Pascual, G.; Laranja, J.L.; Ahmed, F.; Amar, E.; De Troch, M.; Bossier, P.; De Schryver, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ludevese-Pascual, G. <i>et al.</i>","Englishabstract":"The effects of dietary poly‐β‐hydroxybutyrate (PHB) on the lipid and fatty acids (FA) in crustaceans were investigated using <i>Artemia</i> as model species. Supplying PHB either in crystalline or amorphous form significantly increased the whole‐body lipid contents of starved <i>Artemia</i>. Co‐supplying dietary PHB with bacterial PHB degrader <i>Comamonas testosteroni</i> at 10<sup>6</sup> cells&nbsp;ml<sup>−1</sup> for 24&nbsp;hr also significantly increased this parameter. These findings have important implications on the lipid‐saving effects of PHB in crustacean tissues. Dietary PHB, however, did not contribute to the increase in docosahexaenoic acid&nbsp;(DHA) of the naturally DHA‐deprived <i>Artemia</i>. Alternative strategy of co‐supplying dietary PHB with highly unsaturated fatty acid (HUFA)‐rich emulsion for 24&nbsp;hr effectively improved the nutritional contents of <i>Artemia</i> and at the same time assured efficient delivery of PHB to the larval predator. According to a <sup>13</sup>C stable isotope tracer study, the significantly higher δ<sup>13</sup>C levels measured in <i>Artemia</i> 2&nbsp;hr postfeeding with <sup>13</sup>C‐labelled <i>R. eutropha</i> DSM545 indicate that PHB offered in natural matrix is rapidly assimilated. Overall, this study demonstrated the lipid‐saving effects of PHB in <i>Artemia</i>. 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