{"refrec":{"BRefID":331240,"RR":"<b>Thomson, B.; Wenley, J.; Lockwood, S.; Twigg, I.; Currie, K.; Herndl, G.J.; Hepburn, C.D.; Baltar, F.</b> (2020). Relative importance of phosphodiesterase vs. phosphomonoesterase (alkaline phosphatase) activities for dissolved organic phosphorus hydrolysis in epi- and mesopelagic waters. <i>Front. Earth Sci. 8</i>: 560893. <a href=\"https://doi.org/10.3389/feart.2020.560893\" target=\"_blank\">https://doi.org/10.3389/feart.2020.560893</a>","BEntID":324851,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Front. Earth Sci. 8</i>: 560893. <a href=\"https://doi.org/10.3389/feart.2020.560893\" target=\"_blank\">https://doi.org/10.3389/feart.2020.560893</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Thomson, B.; Wenley, J.; Lockwood, S.; Twigg, I.; Currie, K.; Herndl, G.J.; Hepburn, C.D.; Baltar, F.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Thomson, B. <i>et al.</i>","Englishabstract":"Marine microbes use extracellular phosphatases to hydrolyze phosphate from    organic matter. Dissolved organic phosphorus (DOP) is typically present in    higher concentrations than phosphate in oceanic surface waters. Yet, the    fate and role of different DOP components, such as phosphomonoester and    phosphodiester, are poorly understood. Most of the investigations on    extracellular enzymatic hydrolysis of marine DOP have focused on    phosphomonoesterase (MEA) activity (i.e., alkaline phosphatase), whereas    phosphodiesterase (DEA) measurements are scarce. This limits our    understanding of the ecological and biogeochemical role of DOP sources    other than P-monoesters in the sea. We determined extracellular MEA and DEA    activities including their cell-free fractions on a bimonthly basis over 14    months in surface and mesopelagic subantarctic waters, thus covering a wide    range of phosphate availability levels (from <0.5 to 2.3 µM). We found    that DEA and MEA exhibit similar hydrolysis rates in surface as well as in    mesopelagic waters. The MEA:DEA ratio varied between 0.38 and 5.42 during    the study period, indicating potential differences in function and/or    expression among the two enzyme groups, potentially reflecting differences    in the availability and/or utilization of P-monoester and P-diester pools.    Interestingly, the MEA:DEA was negatively correlated to phosphate (<i>r</i>    = −0.82, <i>p</i> = 0.02, <i>R</i><sup>2</sup>=0.67) and positively withthe inorganic N:P ratio (<i>r</i> = 0.84, <i>p</i> = 0.02, <i>R</i><sup>2</sup> = 0.67), suggesting that the relative importance of DEA    <i>vs.</i> MEA is linked to inorganic P availability and the N:P ratio. DEA    was also related to the N:P ratio, both at the surface and at depth,    suggesting DEA alone is sensitive to changes in the N:P ratio. The majority    (>70%) of extracellular MEA and DEA was found in the cell-free fraction,    increasing with depth for MEA. Our results indicated that DOP hydrolysis    mediated by DEA in the surface as well as in dark ocean is as important as    the frequently measured MEA.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Relative importance of phosphodiesterase vs. phosphomonoesterase (alkaline phosphatase) activities for dissolved organic phosphorus hydrolysis in epi- and mesopelagic waters","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-11 01:32:42.868826","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"extracellular enzymatic activity; phosphomonoesterase; phosphodiesterase; alkaline phosphatase activity; phosphorus","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-11-20","DateCreate":"2020-11-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000575998300001","VABBcode":null,"OpenAcc":1,"DOI":"10.3389/feart.2020.560893"},"refs":null,"anarec":{"AnaID":331240,"PubliDate":2020,"Pagination":"560893","XtraPublOfAnaID":null,"ISBN":null,"Volume":"8","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":286286,"SerRR":"Frontiers in Earth Science. 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