{"refrec":{"BRefID":331874,"RR":"<b>Jacobs, P.; Greeve, Y.; Sikkema, M.; Dubbeldam, M.; Philippart, C.J.M.</b> (2020). Successful rearing of <i>Ostrea edulis</i> from parents originating from the Wadden Sea, the Netherlands. <i>Aquaculture Reports 18</i>: 100537. <a href=\"https://doi.org/10.1016/j.aqrep.2020.100537\" target=\"_blank\">https://doi.org/10.1016/j.aqrep.2020.100537</a>","BEntID":325486,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquaculture Reports 18</i>: 100537. <a href=\"https://doi.org/10.1016/j.aqrep.2020.100537\" target=\"_blank\">https://doi.org/10.1016/j.aqrep.2020.100537</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Jacobs, P.; Greeve, Y.; Sikkema, M.; Dubbeldam, M.; Philippart, C.J.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Jacobs, P. <i>et al.</i>","Englishabstract":"Since 2017, the European flat oyster (<em>Ostrea edulis</em>) reappeared in the Wadden Sea after decades of absence. In several countries, restoration and reinforcement projects have been initiated. Better insight in <em>Ostrea</em> behaviour is needed to ensure sustainable conservation in the future. In order to acquire substantial numbers of oysters for eco-physiological and transplant experiments without threatening the small local population, indoor rearing facilities were set-up at Royal NIOZ. Here, a broodstock of 38 adults originating from the western Wadden Sea, produced over 21 million larvae in 10 batches within 3 months (Sept-Nov 2018). Release of larvae and rearing took place at a water temperature of 19.7 ± 0.6 °C and a salinity of 30.5 ± 0.5 PSU. Outbreaks of <em>Vibrio</em> bacteria did not occur. The growth rate before settlement was 1.5–1.7 μm per day, which was at the low end of growth rates reported in previous studies. The first competent larvae were observed 17–22 days after release. Size at settlement varied between 255−325 μm. Survival until settlement was 0.96−0.98 per day. After settlement, growth rates increased to 30−47 μm per day, with batches that were released in September having a significantly higher growth rate and initial size compared to larvae that were released later. Mortality from release to the end of the experiment (Feb 2019) was 0.04−0.05 per day. A cryopreservation trial did not result in living larvae after thawing, most likely due to their advanced development when preserved. The larvae and juveniles were negatively tested for the parasite <em>Bonamia ostrea</em> and can thus be used in oyster conservation and restoration projects. Recommendations for future breeding attempts include the use of a higher water temperature and slightly higher food concentrations.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Successful rearing of <i>Ostrea edulis</i> from parents originating from the Wadden Sea, the Netherlands","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Coastal; Biodiversity; Endangered species; Reproduction; Benthos; Aquaculture","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2021-01-06","DateCreate":"2020-12-10","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000603384800028","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.aqrep.2020.100537"},"refs":null,"anarec":{"AnaID":331874,"PubliDate":2020,"Pagination":"100537","XtraPublOfAnaID":null,"ISBN":null,"Volume":"18","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":329899,"SerRR":"Aquaculture Reports. 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