{"refrec":{"BRefID":33206,"RR":"<b>Winzer, K.; Van Noorden, C.J.F.; Köhler, A.</b> (2002). Sex-specific biotransformation and detoxification after xenobiotic exposure of primary cultured hepatocytes of European flounder (<i>Platichthys flesus</i> L.). <i>Aquat. Toxicol. 59(1-2)</i>: 17-33. <a href=\"https://dx.doi.org/10.1016/S0166-445X(01)00213-2\" target=\"_blank\">https://dx.doi.org/10.1016/S0166-445X(01)00213-2</a>","BEntID":33206,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 59(1-2)</i>: 17-33. <a href=\"https://dx.doi.org/10.1016/S0166-445X(01)00213-2\" target=\"_blank\">https://dx.doi.org/10.1016/S0166-445X(01)00213-2</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Winzer, K.; Van Noorden, C.J.F.; Köhler, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Winzer, K.; Van Noorden, C.J.F.; Köhler, A.","Englishabstract":"Sex-specific effects of sublethal concentrations of known effective pro-oxidants such as 100, 200 and 400 µM benzo[a]pyrene (B[<i>a</i>]p), 50 µM nitrofurantoin (NF) and 100 µM hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) on biotransformation pathways were studied in isolated hepatocytes of immature female and male European flounder (<i>Platichthys flesus</i> L.). Cell responses were assessed at the level of: (1) stress induction as measured by formation of reactive oxygen species (ROS), mainly superoxide radicals, and induction of cytochrome P450 (CYP450) biotransformation activity; (2) cellular antioxidant defences, both non-enzymatic (reduced glutathione) and enzymatic (DT-diaphorase (DTD) or quinone oxidoreductase, EC 1.6.99.2); (3) detoxification (aldehyde dehydrogenase (ALDH), EC 1.2.1.3); and (4) cellular damage as measured by reduced lysosomal membrane stability and cell death. As there is increasing evidence that 17-β-estradiol interferes with certain pathways of xenobiotic biotransformation, we additionally tested the effects of different concentrations of 17-β-estradiol (0.2-10 µM) alone and 17-β-estradiol (1 µM) in combination with 100 µM B[<i>a</i>]p. Parameters were monitored after 1 and 9 days of exposure by quantitative image analysis of chromogenic or fluorogenic reaction products. Our study revealed sex-dependent differences in cellular stress responses. In hepatocytes of female flounder, biotransformation was slower and the capacity of non-enzymatic antioxidant defences and detoxification of toxic aldehydes was lower than in males. Additional administration of 17-β-estradiol enlarged these differences between the sexes with respect to biotransformation activity and antioxidant defence in xenobiotic-induced injury. These findings may explain the higher susceptibility of female flounder to toxic and carcinogenic compounds in the marine environment.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Sex-specific biotransformation and detoxification after xenobiotic exposure of primary cultured hepatocytes of European flounder (<i>Platichthys flesus</i> L.)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-23 01:31:07.646596","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":"Primary flounder hepatocyte culture; Biotransformation; CYP450; Oxidative stress; Antioxidative defence; Hydrogen peroxide; Benzo[a]pyrene; Nitrofurantoin; Estrogen; ALDH; DTD","Notes":null,"AnaPub":2002,"MonPub":null,"DateUpdate":"2003-03-17","DateCreate":"2003-03-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000176782000002","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/S0166-445X(01)00213-2"},"refs":null,"anarec":{"AnaID":33206,"PubliDate":2002,"Pagination":"17-33","XtraPublOfAnaID":null,"ISBN":null,"Volume":"59","Issue":"1-2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. 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