{"refrec":{"BRefID":33258,"RR":"<b>Winzer, K.; Van Noorden, C.J.F.; Köhler, A.</b> (2002). Glucose-6-phosphate dehydrogenase: the key to sex-related xenobiotic toxicity in hepatocytes of European flounder (<i>Platichthys flesus</i> L.)? <i>Aquat. Toxicol. 56(4)</i>: 275-288. <a href=\"https://dx.doi.org/10.1016/S0166-445X(01)00215-6\" target=\"_blank\">https://dx.doi.org/10.1016/S0166-445X(01)00215-6</a>","BEntID":33258,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":" <i>Aquat. Toxicol. 56(4)</i>: 275-288. <a href=\"https://dx.doi.org/10.1016/S0166-445X(01)00215-6\" target=\"_blank\">https://dx.doi.org/10.1016/S0166-445X(01)00215-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Winzer, K.; Van Noorden, C.J.F.; Köhler, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Winzer, K.; Van Noorden, C.J.F.; Köhler, A.","Englishabstract":"The role of glucose-6-phosphate dehydrogenase (G6PDH) in oxidative stress responses was investigated in isolated intact living hepatocytes of immature female and male European flounder (<i>Platichthys flesus</i> L.) because it is the major provider of NADPH needed as reducing power for various detoxification pathways. Hepatocytes were exposed to sublethal concentrations of effective prooxidants such as 100 μM hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), 100, 200 and 400 μM benzo[a]pyrene (B[a]p) and 50 μM nitrofurantoin (NF) during culture. Since there is evidence that 17-β-estradiol inhibits certain pathways of xenobiotic biotransformation, we tested also the effects of different concentrations of 17-β-estradiol (0.2, 1 and 2 μM) alone and 1 μM in combination with 100 μM B[a]p on G6PDH activity. After short-term (1 day) and long-term (9 days) exposure, G6PDH activity was quantified in intact living hepatocytes by a tetrazolium salt method using tetranitroblue tetrazolium salt (TNBT). Hepatocytes obtained from male fish generally showed higher G6PDH activity than those of females. We observed significant inhibition of G6PDH activity by all oxidative stressors and 17-β-estradiol in both sexes of fish independently of culture conditions, but inhibition was stronger in cells of females than in cells of males. A cumulative effect of the steroid and B[a]p was not found. Our results indicate a sex-dependent inhibitory effect of all stressors and 17-β-estradiol on G6PDH activity in flounder hepatocytes independent of prooxidant activity of the specific compound. Consequently, NADPH supply for xenobiotic detoxification and other cellular antioxidative defence mechanisms may be different in livers of female and male flounder. The strongly decreased supply of NADPH in hepatocytes of females may explain the reduced and/or delayed NADPH-dependent activity of xenobiotic biotransformation systems such as cytochrome P450 (CYP450) and a lower capacity of non-enzymatic defence systems such as reduced glutathione that is particularly observed in female flounder. Moreover, the strong inhibition of G6PDH in livers of female flounder may explain higher susceptibility for xenobiotic toxicity and, therefore, potentially a higher risk to develop liver cancer.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Glucose-6-phosphate dehydrogenase: the key to sex-related xenobiotic toxicity in hepatocytes of European flounder (<i>Platichthys flesus</i> L.)?","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-30 01:30:59.843834","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":"G6PDH; Tetrazolium salt method; Primary hepatocyte culture; Antioxidative response; Biotransformation; Hydrogen peroxide; Benzo[a]pyrene; Nitrofurantoin; Estrogen; Image analysis","Notes":null,"AnaPub":2002,"MonPub":null,"DateUpdate":"2003-03-18","DateCreate":"2003-03-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000174176100004","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/S0166-445X(01)00215-6"},"refs":null,"anarec":{"AnaID":33258,"PubliDate":2002,"Pagination":"275-288","XtraPublOfAnaID":null,"ISBN":null,"Volume":"56","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":275,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Winzer","Firstname":"Katja","Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":33258,"AutID":35758,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Van Noorden","Firstname":"Cornelis","Initials":"C.J.F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":33258,"AutID":35759,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Köhler","Firstname":"Angela","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":33258,"AutID":35760,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; New York; London; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Image analysis","ThestID":152989,"Acronym":"CAB","ThesTermPath":"Analysis > Image analysis"},{"ThesaurusTerm":"Biotransformation","ThestID":60851,"Acronym":"CSA","ThesTermPath":"Biotransformation"},{"ThesaurusTerm":"Hydrogen","ThestID":4099,"Acronym":"ASFA","ThesTermPath":"Chemical elements > Nonmetals > Atmospheric gases > Hydrogen"},{"ThesaurusTerm":"Oxidation","ThestID":5919,"Acronym":"ASFA","ThesTermPath":"Chemical reactions > Oxidation"},{"ThesaurusTerm":"Nitrofurantoin","ThestID":112181,"Acronym":"CAB","ThesTermPath":"Chemicals > Organic compounds > Heterocyclic oxygen compounds > Furans > Nitrofurans > Antibacterial agents > Nitrofurantoin"},{"ThesaurusTerm":"Fish culture","ThestID":3152,"Acronym":"ASFA","ThesTermPath":"Cultures > Fish culture"},{"ThesaurusTerm":"Detoxification","ThestID":2270,"Acronym":"ASFA","ThesTermPath":"Detoxification"},{"ThesaurusTerm":"Hydrogen peroxide","ThestID":74311,"Acronym":"CSA","ThesTermPath":"Hydrogen peroxide"},{"ThesaurusTerm":"Image analysis","ThestID":79706,"Acronym":"CSA","ThesTermPath":"Image analysis"},{"ThesaurusTerm":"Image analysis","ThestID":94316,"Acronym":"TRT","ThesTermPath":"Image interpretation > Image analysis"},{"ThesaurusTerm":"Hydrogen peroxide","ThestID":144344,"Acronym":"CAB","ThesTermPath":"Inorganic compounds > Peroxides > Oxidants > Hydrogen peroxide"},{"ThesaurusTerm":"Hydrogen peroxide","ThestID":99354,"Acronym":"TRT","ThesTermPath":"Peroxide > Hydrogen peroxide"},{"ThesaurusTerm":"Sex hormones","ThestID":7526,"Acronym":"ASFA","ThesTermPath":"Secretory products > Hormones > Sex hormones"}],"thestermsASFA":[{"ThesaurusTerm":"Biotransformation"},{"ThesaurusTerm":"Detoxification"},{"ThesaurusTerm":"Fish culture"},{"ThesaurusTerm":"Hydrogen"},{"ThesaurusTerm":"Hydrogen peroxide"},{"ThesaurusTerm":"Hydrogen peroxide"},{"ThesaurusTerm":"Hydrogen peroxide"},{"ThesaurusTerm":"Image analysis"},{"ThesaurusTerm":"Image analysis"},{"ThesaurusTerm":"Image analysis"},{"ThesaurusTerm":"Nitrofurantoin"},{"ThesaurusTerm":"Oxidation"},{"ThesaurusTerm":"Sex hormones"}],"taxtermsASFA":[{"TaxTerm":"Platichthys flesus (Linneaus, 1758)"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":33258,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/S0166-445X(01)00215-6","externalID":"10.1016/S0166-445X(01)00215-6","URLTypeCode":"DOI","URLID":110527,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Biotransformation","ThestID":60851,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Detoxification","ThestID":2270,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Fish culture","ThestID":3152,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Hydrogen","ThestID":4099,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Hydrogen peroxide","ThestID":74311,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Hydrogen peroxide","ThestID":99354,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"},{"ThesaurusTerm":"Hydrogen peroxide","ThestID":144344,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Image analysis","ThestID":152989,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Image analysis","ThestID":79706,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Image analysis","ThestID":94316,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"},{"ThesaurusTerm":"Nitrofurantoin","ThestID":112181,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Oxidation","ThestID":5919,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Sex hormones","ThestID":7526,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Platichthys flesus (Linneaus, 1758) [Fluke]","AphiaID":127141,"TaxtID":12179}],"geoterms":null,"othterms":[{"OtherTerm":"Antioxidative response","OthtID":9399},{"OtherTerm":"Benzo[a]pyrene","OthtID":2305},{"OtherTerm":"Estrogen","OthtID":7970},{"OtherTerm":"G6pdh","OthtID":3137},{"OtherTerm":"Primary hepatocyte culture","OthtID":8726},{"OtherTerm":"Tetrazolium salt method","OthtID":7141}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Biotransformation","DutchTerm":null,"ThestID":60851,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Detoxification","DutchTerm":"Ontgifting","ThestID":2270,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Fish culture","DutchTerm":null,"ThestID":3152,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Hydrogen","DutchTerm":"Waterstof","ThestID":4099,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Hydrogen peroxide","DutchTerm":null,"ThestID":74311,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Hydrogen peroxide","DutchTerm":null,"ThestID":99354,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"},{"ThesaurusTerm":"Hydrogen peroxide","DutchTerm":null,"ThestID":144344,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Image analysis","DutchTerm":null,"ThestID":152989,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Image analysis","DutchTerm":null,"ThestID":79706,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Image analysis","DutchTerm":null,"ThestID":94316,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"},{"ThesaurusTerm":"Nitrofurantoin","DutchTerm":null,"ThestID":112181,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Oxidation","DutchTerm":"Oxidatie","ThestID":5919,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Sex hormones","DutchTerm":"Geslachtshormonen","ThestID":7526,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Platichthys flesus (Linneaus, 1758)","DutchTerm":"Bot","AphiaID":127141,"TaxtID":12179}],"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"Antioxidative response","DutchTerm":null,"OthtID":9399},{"OtherTerm":"Benzo[a]pyrene","DutchTerm":null,"OthtID":2305},{"OtherTerm":"Estrogen","DutchTerm":null,"OthtID":7970},{"OtherTerm":"G6pdh","DutchTerm":null,"OthtID":3137},{"OtherTerm":"Primary hepatocyte culture","DutchTerm":null,"OthtID":8726},{"OtherTerm":"Tetrazolium salt method","DutchTerm":null,"OthtID":7141}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2003-03-18 14:18:14.013000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2003-03-18 14:18:14.013000","timezone_type":3,"timezone":"Europe/Brussels"}}}