{"refrec":{"BRefID":332604,"RR":"<b>Levy, S.; Brekhman, V.; Bakhman, A.; Malik, A.; Sebé-Pedrós, A.; Kosloff, M.; Lotan, T.</b> (2021). Ectopic activation of GABA<sub>B</sub> receptors inhibits neurogenesis and metamorphosis in the cnidarian <i>Nematostella vectensis</i>. <i>Nature Ecology & Evolution 5(1)</i>: 111-121. <a href=\"https://dx.doi.org/10.1038/s41559-020-01338-3\" target=\"_blank\">https://dx.doi.org/10.1038/s41559-020-01338-3</a>","BEntID":326216,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Nature Ecology & Evolution 5(1)</i>: 111-121. <a href=\"https://dx.doi.org/10.1038/s41559-020-01338-3\" target=\"_blank\">https://dx.doi.org/10.1038/s41559-020-01338-3</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Levy, S.; Brekhman, V.; Bakhman, A.; Malik, A.; Sebé-Pedrós, A.; Kosloff, M.; Lotan, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Levy, S. <i>et al.</i>","Englishabstract":"The metabotropic gamma-aminobutyric acid B receptor (GABA<sub>B</sub>R) is a G protein-coupled receptor that mediates neuronal inhibition by the neurotransmitter GABA. While GABA<sub>B</sub>R-mediated signalling has been suggested to play central roles in neuronal differentiation and proliferation across evolution, it has mostly been studied in the mammalian brain. Here, we demonstrate that ectopic activation of GABA<sub>B</sub>R signalling affects neurogenic functions in the sea anemone <i>Nematostella vectensis</i>. We identified four putative <i>Nematostella</i> GABA<sub>B</sub>R homologues presenting conserved three-dimensional extracellular domains and residues needed for binding GABA and the GABA<sub>B</sub>R agonist baclofen. Moreover, sustained activation of GABA<sub>B</sub>R signalling reversibly arrests the critical metamorphosis transition from planktonic larva to sessile polyp life stage. To understand the processes that underlie the developmental arrest, we combined transcriptomic and spatial analyses of control and baclofen-treated larvae. Our findings reveal that the cnidarian neurogenic programme is arrested following the addition of baclofen to developing larvae. Specifically, neuron development and neurite extension were inhibited, resulting in an underdeveloped and less organized nervous system and downregulation of proneural factors including <i>NvSoxB(2)</i>, <i>NvNeuroD1</i> and <i>NvElav1</i>. Our results thus point to an evolutionarily conserved function of GABA<sub>B</sub>R in neurogenesis regulation and shed light on early cnidarian development.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Ectopic activation of GABA<sub>B</sub> receptors inhibits neurogenesis and metamorphosis in the cnidarian <i>Nematostella vectensis</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-01-12","DateCreate":"2021-01-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000587977100002","VABBcode":null,"OpenAcc":0,"DOI":"10.1038/s41559-020-01338-3"},"refs":null,"anarec":{"AnaID":332604,"PubliDate":2021,"Pagination":"111-121","XtraPublOfAnaID":null,"ISBN":null,"Volume":"5","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":282823,"SerRR":"Nature Ecology & Evolution. 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