{"refrec":{"BRefID":334913,"RR":"<b>Tourneroche, A.; Lami, R.; Burgaud, G.; Domart-Coulon, I.; Li, W.; Gachon, C.; Gèze, M.; Boeuf, D.; Prado, S.</b> (2020). The bacterial and fungal microbiota of <i>Saccharina latissima</i> (Laminariales, Phaeophyceae). <i>Front. Mar. Sci. 7</i>: 587566. <a href=\"https://dx.doi.org/10.3389/fmars.2020.587566\" target=\"_blank\">https://dx.doi.org/10.3389/fmars.2020.587566</a>","BEntID":331470,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Front. Mar. Sci. 7</i>: 587566. <a href=\"https://dx.doi.org/10.3389/fmars.2020.587566\" target=\"_blank\">https://dx.doi.org/10.3389/fmars.2020.587566</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tourneroche, A.; Lami, R.; Burgaud, G.; Domart-Coulon, I.; Li, W.; Gachon, C.; Gèze, M.; Boeuf, D.; Prado, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tourneroche, A. <i>et al.</i>","Englishabstract":"The sugar kelp <i>Saccharina latissima</i> dominates many temperate coastal ecosystems, plays key ecological roles and presents important economic potential. However, its microbiota remains poorly investigated, although it could play an important role in algal fitness. In this study, we combined high throughput Illumina-based DNA sequencing and Fluorescence <i>In Situ</i> Hybridization to perform a culture-independent investigation of the <i>S. latissima</i> bacterial and fungal microbiota. Up to 600 bacterial and 100 fungal Amplicon Sequence Variants were identified per algal individual, revealing diverse bacterial and fungal communities associated to <i>S. latissima</i>. Overall, bacterial communities were dominated by Proteobacteria, Actinobacteria, and Bacteroidetes, in particular <i>Hyphomonadaceae</i> and <i>Cyclobacteriaceae</i>. Fungal communities were dominated by <i>Ascomycota</i> and <i>Basidiomycota</i>, in particular <i>Mycosphaerellaceae, Psathyrellaceae</i>, and <i>Bulleribasidiaceae</i>. Our results also revealed a variable distribution of <i>S. latissima</i> microbiota, as two adjacent tissue samples typically contained distinct fungal and bacterial assemblages, and CARD-FISH analysis detected microbial endosymbionts (with a few epibionts). Complementary analyses showed that despite achieving a good sequencing coverage for each tissue sample, the unexpected diversity and variability of ASVs made the definition of a core fungal and bacterial microbiota difficult, and highlights novel avenues to overcome the limitations of current surface-sterilization and metabarcoding protocols.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The bacterial and fungal microbiota of <i>Saccharina latissima</i> (Laminariales, Phaeophyceae)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-02 01:32:05.553892","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"algae, fungi, bacteria, holobiont, endophyte, biofilm, microbiota, Saccharina latissima","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2021-03-17","DateCreate":"2021-03-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000602446100001","VABBcode":null,"OpenAcc":1,"DOI":"10.3389/fmars.2020.587566"},"refs":null,"anarec":{"AnaID":334913,"PubliDate":2020,"Pagination":"587566","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":233602,"SerRR":"Frontiers in Marine Science. 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