{"refrec":{"BRefID":334936,"RR":"<b>Polerecky, L.; Masuda, T.; Eichner, M.; Rabouille, S.; Vancová, M.; Kienhuis, M.V.M.; Bernát, G.; Bonomi-Barufi, J.; Campbell, D.A.; Claquin, P.; Červený, J.; Giordano, M.; Kotabová, E.; Kromkamp, J.; Lombardi, A.T.; Lukeš, M.; Prášil, O.; Stephan, S.; Suggett, D.; Zavřel, T.; Halsey, K.H.</b> (2021). Temporal patterns and intra- and inter-cellular variability in carbon and nitrogen assimilation by the unicellular cyanobacterium <i>Cyanothece</i>sp. ATCC 51142. <i>Front. Microbiol. 12</i>: 620915. <a href=\"https://doi.org/10.3389/fmicb.2021.620915\" target=\"_blank\">https://doi.org/10.3389/fmicb.2021.620915</a>","BEntID":331493,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Front. Microbiol. 12</i>: 620915. <a href=\"https://doi.org/10.3389/fmicb.2021.620915\" target=\"_blank\">https://doi.org/10.3389/fmicb.2021.620915</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Polerecky, L.; Masuda, T.; Eichner, M.; Rabouille, S.; Vancová, M.; Kienhuis, M.V.M.; Bernát, G.; Bonomi-Barufi, J.; Campbell, D.A.; Claquin, P.; Červený, J.; Giordano, M.; Kotabová, E.; Kromkamp, J.; Lombardi, A.T.; Lukeš, M.; Prášil, O.; Stephan, S.; Suggett, D.; Zavřel, T.; Halsey, K.H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Polerecky, L. <i>et al.</i>","Englishabstract":"<p>    Unicellular nitrogen fixing cyanobacteria (UCYN) are abundant members of    phytoplankton communities in a wide range of marine environments, including    those with rapidly changing nitrogen (N) concentrations. We hypothesized    that differences in N availability (N<sub>2</sub> vs. combined N) would    cause UCYN to shift strategies of intracellular N and C allocation. We used    transmission electron microscopy and nanoscale secondary ion massspectrometry imaging to track assimilation and intracellular allocation of<sup>13</sup>C-labeled CO<sub>2</sub> and <sup>15</sup>N-labeled N<sub>2</sub> or NO<sub>3</sub> at different periods across a diel cycle in    <em>Cyanothece</em> sp. ATCC 51142. We present new ideas on interpreting    these imaging data, including the influences of pre-incubation cellular C    and N contents and turnover rates of inclusion bodies. Within cultures    growing diazotrophically, distinct subpopulations were detected that fixed    N<sub>2</sub> at night or in the morning. Additional significant    within-population heterogeneity was likely caused by differences in the    relative amounts of N assimilated into cyanophycin from sources externaland internal to the cells. Whether growing on N<sub>2</sub> or NO    <sub>3</sub>, cells prioritized cyanophycin synthesis when N assimilation    rates were highest. N assimilation in cells growing on NO<sub>3</sub>    switched from cyanophycin synthesis to protein synthesis, suggesting that    once a cyanophycin quota is met, it is bypassed in favor of protein    synthesis. Growth on NO<sub>3</sub> also revealed that at night, there is a    very low level of CO<sub>2</sub> assimilation into polysaccharides    simultaneous with their catabolism for protein synthesis. This studyrevealed multiple, detailed mechanisms underlying C and N management in    <em>Cyanothece</em> that facilitate its success in dynamic aquatic    environments.</p>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Temporal patterns and intra- and inter-cellular variability in carbon and nitrogen assimilation by the unicellular cyanobacterium <i>Cyanothece</i>sp. ATCC 51142","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:32:09.907843","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<i>Crocosphaera subtropica</i> (former <i>Cyanothece</i> sp. 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