{"refrec":{"BRefID":336934,"RR":"<b>Smitz, N.; De wolf, K.; Gheysen, A.; Deblauwe, I.; Meganck, K.; De Witte, J.; Schneider, A.; Verlé, I.; Dekoninck, W.; Gombeer, S.; Vanderheyden, A.; De Meyer, M.; Backeljau, T.; Müller, R.; Van Bortel, W.</b> (2021). DNA identification of species of the <i>Anopheles maculipennis</i> complex and first record of <i>An. daciae</i> in Belgium. <i>Medical and Veterinary Entomology 35(3)</i>: 442-450. <a href=\"https://hdl.handle.net/10.1111/mve.12519\" target=\"_blank\">https://hdl.handle.net/10.1111/mve.12519</a>","BEntID":333556,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Medical and Veterinary Entomology 35(3)</i>: 442-450. <a href=\"https://hdl.handle.net/10.1111/mve.12519\" target=\"_blank\">https://hdl.handle.net/10.1111/mve.12519</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Smitz, N.; De wolf, K.; Gheysen, A.; Deblauwe, I.; Meganck, K.; De Witte, J.; Schneider, A.; Verlé, I.; Dekoninck, W.; Gombeer, S.; Vanderheyden, A.; De Meyer, M.; Backeljau, T.; Müller, R.; Van Bortel, W.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Smitz, N. <i>et al.</i>","Englishabstract":"The present study aimed at identifying the members of the <i>Anopheles maculipennis</i> complex (Diptera: Culicidae) occurring in Belgium. Therefore, the second internal transcribed spacer of nuclear ribosomal DNA (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI) loci were sequenced in 175 and 111 specimens, respectively, collected between 2007 and 2019. In parallel, the suitability of two species‐diagnostic PCR‐RFLP assays was tested. The identified specimens included: <i>An. maculipennis</i> s.s. (N = 105), <i>An. daciae</i> (N = 62), <i>An. atroparvus</i> (N = 6) and <i>An. messeae</i> (N = 2). Each species was characterized by unique ITS2 haplotypes, whereas COI only supported the monophyly of <i>An. atroparvus</i>, a historical malaria vector in Belgium. Species identification results were further supported by unique PCR‐RFLP banding patterns. We report for the first time <i>An. daciae</i> in Belgium, where it was found to co‐occur with <i>An. maculipennis</i> s.s. The latter was the most prevalent in the collection studied (60%) and appears to have the widest distribution in Belgium<i>.</i> As in other studies, <i>An. daciae</i> and <i>An. messeae</i> appeared the most closely related species, up to the point that their species status remains debatable, while their ecological differences, including vector competences, need further study.","AbstractOtherLang":"Anopheles maculipennis subgroup, cytochrome oxidase I (COI), internaltranscribed spacer 2 (ITS2), malaria vector, occurrence, restriction fragment length polymorphism (RFL","BibLvlCode":"AS","StandardTitle":"DNA identification of species of the <i>Anopheles maculipennis</i> complex and first record of <i>An. daciae</i> in Belgium","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Anopheles maculipennis subgroup, cytochrome oxidase I (COI), internal transcribed spacer 2 (ITS2), malaria vector, occurrence, restriction fragment length polymorphism (RFL","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-08-17","DateCreate":"2021-05-06","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000647204800001","VABBcode":null,"OpenAcc":1,"Handle":"10.1111/mve.12519"},"refs":null,"anarec":{"AnaID":336934,"PubliDate":2021,"Pagination":"442-450","XtraPublOfAnaID":null,"ISBN":null,"Volume":"35","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":274499,"SerRR":"Medical and Veterinary Entomology. 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