{"refrec":{"BRefID":337275,"RR":"<b>Loor, A.; Bossier, P.; Nevejan, N.</b> (2021). Dietary substitution of microalgae with the <i>Saccharomyces cerevisiae</i> mutant, Δmnn9, for feeding Pacific oyster (<i>Crassostrea gigas</i>) juveniles. <i>Aquaculture 534</i>: 736253. <a href=\"https://hdl.handle.net/10.1016/j.aquaculture.2020.736253\" target=\"_blank\">https://hdl.handle.net/10.1016/j.aquaculture.2020.736253</a>","BEntID":333897,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquaculture 534</i>: 736253. <a href=\"https://hdl.handle.net/10.1016/j.aquaculture.2020.736253\" target=\"_blank\">https://hdl.handle.net/10.1016/j.aquaculture.2020.736253</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Loor, A.; Bossier, P.; Nevejan, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Loor, A.; Bossier, P.; Nevejan, N.","Englishabstract":"Microalgae production is one of the major limiting factors for bivalve aquaculture due to its high production costs, risks of contamination, and nutritional variability between algal species. Alternative diets to live microalgae have been tested in bivalves, but no satisfactory products have been developed that complete their nutritional requirements. The baker's yeast (<em>Saccharomyces cerevisiae</em>) represents a potential candidate, however, its poor digestibility, partly associated to the high mannan content in the cell wall, limits its use in aquaculture. This study evaluated the use of the <em>S. cerevisiae</em> mnn9 mutant (Δ<em>mnn9</em>), whose mutation results in a truncation of the mannan structure, to substitute microalgal diets for feeding Pacific oyster (<em>Crassostrea gigas</em>) spat. First, the optimal feeding ration of a bi-algal diet (<em>Chaetoceros muelleri</em> and <em>Tisochrysis lutea</em>, 50:50 based on dry weight (DW)) was determined (trial 1). Then, the replacement of the microalgal diet with Δ<em>mnn9</em> was evaluated at five substitution levels (0, 25, 50, 75, and 100%, based on DW; trial 2). Finally, the nutritional value of Δ<em>mnn9</em> was compared with the wild-type <em>S. cerevisiae</em> by replacing 50% (DW) of the algal diet with either Δ<em>mnn9</em> or the wild type (trial 3). Gene expressions of elongation of very-long-chain fatty acids (<em>Cg-</em>ELOVL) and β-glucan-binding protein (<em>Cg-</em>βGBP) were measured in trial 2 and trial 3, respectively. Trial 1: the lowest daily feeding ration in attaining the highest significant specific growth rate (SGR) was 2% (SGR: 7.6% day<sup>−1</sup>). Trial 2: Δ<em>mnn9</em> could substitute 50% (DW) of the microalgal diet without significantly affecting the general performance of the spat. Up-regulation of <em>Cg-</em>ELOVL mRNA was observed in oysters fed the 75% substitution diet compared with those fed 100% algae. Trial 3: significant growth improvement was observed in oysters fed Δ<em>mnn9</em> compared with those fed wild type<em>.</em> Higher gene expression of <em>Cg-</em>βGBP was observed with the Δ<em>mnn9</em>-containing diet than with the wild-type diet, suggesting immunostimulatory properties of Δ<em>mnn9</em> cells. These results indicate that Δ<em>mnn9</em> cells are efficiently ingested and digested by <em>C. gigas</em> spat, and represents a suitable alternative to a microalgal diet in this species. Fatty acid elongases seem to participate in the biosynthetic pathways of polyunsaturated fatty acids (PUFAs) in <em>C. gigas</em> and their activity could increase as a response to PUFA deficiencies in the diet.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Dietary substitution of microalgae with the <i>Saccharomyces cerevisiae</i> mutant, Δmnn9, for feeding Pacific oyster (<i>Crassostrea gigas</i>) juveniles","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Pacific oyster; Crassostrea gigas; Algal replacement; Mutant yeast; Fatty acid elongase; ß-Glucan-binding protein","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-05-20","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000614763900004","VABBcode":null,"OpenAcc":0,"Handle":"10.1016/j.aquaculture.2020.736253"},"refs":null,"anarec":{"AnaID":337275,"PubliDate":2021,"Pagination":"736253","XtraPublOfAnaID":null,"ISBN":null,"Volume":"534","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo.  ISSN 0044-8486; e-ISSN 1873-5622","StandardTitleSer":"Aquaculture","ISSN":"0044-8486","AbbrevSer":"Aquaculture","StandardTitleMon":null,"StartPage":12,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Loor","Firstname":"Alfredo","Initials":"A.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":333897,"AutID":445540,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-9265-5367","PersID":40045,"InsID":22},{"AutName":"Bossier","Firstname":"Peter","Initials":"P.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":333897,"AutID":144468,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-6165-9111","PersID":125,"InsID":22},{"AutName":"Nevejan","Firstname":"Nancy","Initials":"N.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":333897,"AutID":189074,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0001-6566-4607","PersID":15522,"InsID":22}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":361339,"LibID":36,"BRefID":337275,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"361339","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Amsterdam; London; New York; Oxford; Tokyo"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Crassostrea gigas"},{"TaxTerm":"Saccharomyces cerevisiae"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":333897,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":333897,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://hdl.handle.net/10.1016/j.aquaculture.2020.736253","externalID":"10.1016/j.aquaculture.2020.736253","URLTypeCode":"Handle","URLID":92940,"URLTypID":32,"URLType":"Handle","URLPrefix":"https://hdl.handle.net/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Crassostrea gigas [Portuguese oyster]","AphiaID":836033,"TaxtID":22937},{"TaxTerm":"Saccharomyces cerevisiae","AphiaID":147593,"TaxtID":34632}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Crassostrea gigas","DutchTerm":"Japanse oester","AphiaID":836033,"TaxtID":22937},{"TaxTerm":"Saccharomyces cerevisiae","DutchTerm":"bakkersgist","AphiaID":147593,"TaxtID":34632}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2021-05-17 16:42:41.567000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2021-05-20 08:21:48.877000","timezone_type":3,"timezone":"Europe/Brussels"}}}