{"refrec":{"BRefID":337300,"RR":"<b>Fu, S.; Ni, P.; Yang, Q.; Hu, H.; Wang, Q.; Ye, S.; Liu, Y.</b> (2021). Delineating the key virulence factors and intraspecies divergence of <i>Vibrio harveyi</i> via whole-genome sequencing. <i>Can. J. Microbiol. 67(3)</i>: 231-248. <a href=\"https://hdl.handle.net/10.1139/cjm-2020-0079\" target=\"_blank\">https://hdl.handle.net/10.1139/cjm-2020-0079</a>","BEntID":333922,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Can. J. Microbiol. 67(3)</i>: 231-248. <a href=\"https://hdl.handle.net/10.1139/cjm-2020-0079\" target=\"_blank\">https://hdl.handle.net/10.1139/cjm-2020-0079</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Fu, S.; Ni, P.; Yang, Q.; Hu, H.; Wang, Q.; Ye, S.; Liu, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Fu, S. <i>et al.</i>","Englishabstract":"<i>Vibrio harveyi</i> is one of the major pathogens in aquaculture. To identify the key virulence factors affecting pathogenesis of <i>V. harveyi</i> towards fish, we conducted a field investigation for three representative fish farms infected with <i>V. harveyi</i>. Multilocus sequence typing (MLST) and whole-genome sequencing were conducted to delineate the phylogenetic relationship and genetic divergence of <i>V. harveyi</i>. A total of 25 <i>V. harveyi</i> strains were isolated from the diseased fish and groundwater and were subtyped into 12 sequence types by MLST. Five virulence genes, <i>mshB</i>, <i>pilA</i>, <i>hutR</i>, <i>ureB</i>, and <i>ureG</i>, were variably presented in the sequenced strains. The virulence gene profiles strongly correlated with the distinct pathogenicity of <i>V. harveyi</i> strains, with a strain harboring all five genes exhibiting the highest virulence towards fish. Phenotype assay confirmed that reduced virulence correlated with decreased motility and biofilm formation ability. Additionally, three types of type VI secretion system, namely T6SS1, T6SS2, and T6SS3, were identified in <i>V.&nbsp;harveyi</i> strains, which can be classified into six, four, and 12 subtypes, respectively. In conclusion, the results indicated that the virulence level of <i>V.&nbsp;harveyi</i> is mainly determined by the above virulence genes, which may play vital roles in environmental adaptation for <i>V.&nbsp;harveyi.</i>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Delineating the key virulence factors and intraspecies divergence of <i>Vibrio harveyi</i> via whole-genome sequencing","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-25 01:33:17.771283","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Vibrio harveyi; tiger puffer; genetic divergence; type VI secretion system; virulence factors","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-05-24","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000622974500004","VABBcode":null,"OpenAcc":0,"Handle":"10.1139/cjm-2020-0079"},"refs":null,"anarec":{"AnaID":337300,"PubliDate":2021,"Pagination":"231-248","XtraPublOfAnaID":null,"ISBN":null,"Volume":"67","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42536,"SerRR":"Canadian Journal of Microbiology = Journal Canadien de Microbiologie. National Research Council: Ottawa.  ISSN 0008-4166; e-ISSN 1480-3275","StandardTitleSer":"Canadian Journal of Microbiology = Journal Canadien de Microbiologie","ISSN":"0008-4166","AbbrevSer":"Can. J. 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