{"refrec":{"BRefID":337317,"RR":"<b>Hermans, L.; De Pelsmaeker, S.; Denaeghel, S.; Cox, E.; Favoreel, H.W.; Devriendt, B.</b> (2021). β-glucan-induced IL-10 secretion by monocytes triggers porcine NK cell cytotoxicity. <i>Frontiers in Immunology 12</i>: 634402. <a href=\"https://hdl.handle.net/10.3389/fimmu.2021.634402\" target=\"_blank\">https://hdl.handle.net/10.3389/fimmu.2021.634402</a>","BEntID":333939,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Frontiers in Immunology 12</i>: 634402. <a href=\"https://hdl.handle.net/10.3389/fimmu.2021.634402\" target=\"_blank\">https://hdl.handle.net/10.3389/fimmu.2021.634402</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hermans, L.; De Pelsmaeker, S.; Denaeghel, S.; Cox, E.; Favoreel, H.W.; Devriendt, B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hermans, L. <i>et al.</i>","Englishabstract":"Beta-glucans are naturally occurring polysaccharides present in cell walls of fungi, yeast, bacteria, cereals, seaweed, and algae. These microbe-associated molecular patterns (MAMPs) possess immunomodulatory properties. In human, it has been suggested that NK cells can be activated by β-glucans. Here, we aimed to elucidate whether β-glucans modulate porcine NK cell responses <i>in vitro</i> and if so, how these effects are mediated. We investigated the effect of two β-glucans, Macrogard and Curdlan, which differ in solubility and structure. Direct addition of β-glucans to purified porcine NK cells did not affect cytotoxicity of these cells against K562 target cells. However, when using PBMC instead of purified NK cells, β-glucan addition significantly increased NK cell-mediated cytotoxicity. This effect depended on factors secreted by CD14+ monocytes upon β-glucan priming. Further analysis showed that monocytes secrete TNF-α, IL-6, and IL-10 upon β-glucan addition. Of these, IL-10 turned out to play a critical role in β-glucan-triggered NK cell cytotoxicity, since depletion of IL-10 completely abrogated the β-glucan-induced increase in cytotoxicity. Furthermore, addition of recombinant IL-10 to purified NK cells was sufficient to enhance cytotoxicity. In conclusion, we show that β-glucans trigger IL-10 secretion by porcine monocytes, which in turn leads to increased NK cell cytotoxicity, and thereby identify IL-10 as a potent stimulus of porcine NK cell cytotoxicity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"β-glucan-induced IL-10 secretion by monocytes triggers porcine NK cell cytotoxicity","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-09-08","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000625827300001","VABBcode":null,"OpenAcc":1,"Handle":"10.3389/fimmu.2021.634402"},"refs":null,"anarec":{"AnaID":337317,"PubliDate":2021,"Pagination":"634402","XtraPublOfAnaID":null,"ISBN":null,"Volume":"12","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":268438,"SerRR":"Frontiers in Immunology. 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