{"refrec":{"BRefID":337318,"RR":"<b>Guan, A.; Van Damme, I.; Devlieghere, F.; Gabriël, S.</b> (2021). Effect of temperature, CO<sub>2</sub> and O<sub>2</sub> on motility and mobility of Anisakidae larvae. <i>NPG Scientific Reports 11(1)</i>: 4279. <a href=\"https://hdl.handle.net/10.1038/s41598-021-83505-5\" target=\"_blank\">https://hdl.handle.net/10.1038/s41598-021-83505-5</a>","BEntID":333940,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 11(1)</i>: 4279. <a href=\"https://hdl.handle.net/10.1038/s41598-021-83505-5\" target=\"_blank\">https://hdl.handle.net/10.1038/s41598-021-83505-5</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Guan, A.; Van Damme, I.; Devlieghere, F.; Gabriël, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Guan, A. <i>et al.</i>","Englishabstract":"Anisakidae, marine nematodes, are underrecognized fish-borne zoonotic parasites. Studies on factors that could trigger parasites to actively migrate out of the fish are very limited. The objective of this study was to assess the impact of different environmental conditions (temperature, CO<sub>2</sub> and O<sub>2</sub>) on larval motility (in situ movement) and mobility (migration) in vitro. Larvae were collected by candling or enzymatic digestion from infected fish, identified morphologically and confirmed molecularly. Individual larvae were transferred to a semi-solid Phosphate Buffered Saline agar, and subjected to different temperatures (6 ℃, 12 ℃, 22 ℃, 37 ℃) at air conditions. Moreover, different combinations of CO<sub>2</sub> and O<sub>2</sub> with N<sub>2</sub> as filler were tested, at both 6 °C and 12 °C. Video recordings of larvae were translated into scores for larval motility and mobility. Results showed that temperature had significant influence on larval movements, with the highest motility and mobility observed at 22 ℃ for <i>Anisakis</i> spp. larvae and 37 ℃ for <i>Pseudoterranova</i> spp. larvae. During the first 10 min, the median migration of <i>Anisakis</i> spp. larvae was 10 cm at 22 ℃, and the median migration of <i>Pseudoterranova</i> spp. larvae was 3 cm at 37 ℃. Larval mobility was not significantly different under the different CO<sub>2</sub> or O<sub>2</sub> conditions at 6 °C and 12 ℃. It was concluded that temperature significantly facilitated larval movement with the optimum temperature being different for <i>Anisakis</i> spp. and <i>Pseudoterranova</i> spp., while CO<sub>2</sub> and O<sub>2</sub> did not on the short term. This should be further validated in parasite-infected/spiked fish fillets.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effect of temperature, CO<sub>2</sub> and O<sub>2</sub> on motility and mobility of Anisakidae larvae","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:33:00.098277","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-09-09","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000621514400029","VABBcode":null,"OpenAcc":1,"Handle":"10.1038/s41598-021-83505-5"},"refs":null,"anarec":{"AnaID":337318,"PubliDate":2021,"Pagination":"4279","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":11,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Guan","Firstname":"Aiyan","Initials":"A.","Affiliation":"FMFP-UGent, Research Unit Laboratory of Food Microbiology and Food Preservation, Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":333940,"AutID":445951,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"LFMFP","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Levensmiddelentechnologie, Voedselveiligheid en Gezondheid; Laboratorium voor Levensmiddelenmicrobiologie en -conservering","ORCID":null,"PersID":40111,"InsID":7330},{"AutName":"Van Damme","Firstname":"Inge","Initials":"I.","Affiliation":"Laboratory of Foodborne Parasitic Zoonoses, Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":333940,"AutID":445953,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"LFBP","InsFSN":"Universiteit Gent; 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