{"refrec":{"BRefID":337355,"RR":"<b>Hernandez-Balmaseda, I.; Guerra, I.R.; Declerck, K.; Isidron, J.A.H.; Perez-Novo, C.; Van Camp, G.; De Wever, O.; Gonzalez, K.; Labrada, M.; Carr, A.; Dantas-Cassali, G.; dos Reis, D.C.; Delgado-Roche, L.; Nunez, R.R.; Delgado-Hernandez, R.; Fernandez, M.D.; ; Vanden Berghe, W.</b> (2021). Marine seagrass extract of <i>Thalassia testudinum</i> suppresses colorectal tumor growth, motility and angiogenesis by autophagic stress and immunogenic cell death pathways. <i>Mar. Drugs 19(2)</i>: 52. <a href=\"https://hdl.handle.net/10.3390/md19020052\" target=\"_blank\">https://hdl.handle.net/10.3390/md19020052</a>","BEntID":333977,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Drugs 19(2)</i>: 52. <a href=\"https://hdl.handle.net/10.3390/md19020052\" target=\"_blank\">https://hdl.handle.net/10.3390/md19020052</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hernandez-Balmaseda, I.; Guerra, I.R.; Declerck, K.; Isidron, J.A.H.; Perez-Novo, C.; Van Camp, G.; De Wever, O.; Gonzalez, K.; Labrada, M.; Carr, A.; Dantas-Cassali, G.; dos Reis, D.C.; Delgado-Roche, L.; Nunez, R.R.; Delgado-Hernandez, R.; Fernandez, M.D.; ; Vanden Berghe, W.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hernandez-Balmaseda, I. <i>et al.</i>","Englishabstract":"Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm <i>Thalassia testudinum</i> (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dose-dependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC50 values of, respectively, 175, 115, and 60 μg/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NFκB specific gene expression and autophagy stress pathways. 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