{"refrec":{"BRefID":337383,"RR":"<b>Junprung, W.; Supungul, P.; Tassanakajon, A.; Van Stappen, G.; Bossier, P.</b> (2021). Balancing selection at the ATP binding site of heat shock cognate 70 (HSC70) contributes to increased thermotolerance in <i>Artemia franciscana</i>. <i>Aquaculture 531</i>: 735988. <a href=\"https://hdl.handle.net/10.1016/j.aquaculture.2020.735988\" target=\"_blank\">https://hdl.handle.net/10.1016/j.aquaculture.2020.735988</a>","BEntID":334005,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquaculture 531</i>: 735988. <a href=\"https://hdl.handle.net/10.1016/j.aquaculture.2020.735988\" target=\"_blank\">https://hdl.handle.net/10.1016/j.aquaculture.2020.735988</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Junprung, W.; Supungul, P.; Tassanakajon, A.; Van Stappen, G.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Junprung, W. <i>et al.</i>","Englishabstract":"A number of single-nucleotide polymorphisms (SNPs) in heat shock protein 70 (HSP70) family genes have been reported to be associated with stress tolerance. In this study, the presence of SNPs in either the <em>ArHSP70</em> and <em>ArHSC70</em> genes (heat shock cognate gene) was verified in 2 <em>Artemia franciscana</em> populations, either a control population (CF12) or a population selectively bred for increased induced thermotolerance (TF12) over 12 generations. In TF12 animals, a novel non-synonymous SNP was identified at position 171 of the cDNA (C171A; N57K; in the ATP/ADP binding site) in <em>ArHSC70</em> but not in <em>ArHSP70</em>. Cloning and expression of the 2 allelic forms in yeast (<em>Saccharomyces cerevisiae</em>) confirmed that yeast cells containing an <em>Ar</em>HSC70-N57K plasmid could tolerate higher temperatures than yeast cells containing an <em>Ar</em>HSC70-wild type plasmid. This strongly suggests that the SNP at C171A (N57K) increases thermotolerance of the TF12 <em>A. franciscana</em> population. Upon analysis of individuals of the CF12 and TF12 populations it appears that the C171A allele frequency increased substantially in the TF12 population. Both populations appear not to be in Hardy-Weinberg equilibrium, with CF12 displaying an apparent lack of heterozygotes, while the TF12 displays a large excess of heterozygotes. It is argued that these observations are a novel case of balancing selection. As such, HSP genes join a group of immune related genes where balancing selection contributes to population fitness.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Balancing selection at the ATP binding site of heat shock cognate 70 (HSC70) contributes to increased thermotolerance in <i>Artemia franciscana</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Balancing selection; Heat shock cognate 70; Selective breeding; Thermotolerance; Artemia franciscana","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-05-20","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000595053000015","VABBcode":null,"OpenAcc":0,"Handle":"10.1016/j.aquaculture.2020.735988"},"refs":null,"anarec":{"AnaID":337383,"PubliDate":2021,"Pagination":"735988","XtraPublOfAnaID":null,"ISBN":null,"Volume":"531","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. 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