{"refrec":{"BRefID":337472,"RR":"<b>Yang, Q.; Fu, S.; Zou, P.; Hao, J.; Wei, D.; Xie, G.; Huang, J.</b> (2021). Coordination of primary metabolism and virulence factors expression mediates the virulence of <i>Vibrio parahaemolyticus</i> towards cultured shrimp (<i>Penaeus vannamei</i>). <i>J. Appl. Microbiol. 131(1)</i>: 50-67. <a href=\"https://hdl.handle.net/10.1111/jam.14922\" target=\"_blank\">https://hdl.handle.net/10.1111/jam.14922</a>","BEntID":334095,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Appl. Microbiol. 131(1)</i>: 50-67. <a href=\"https://hdl.handle.net/10.1111/jam.14922\" target=\"_blank\">https://hdl.handle.net/10.1111/jam.14922</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Yang, Q.; Fu, S.; Zou, P.; Hao, J.; Wei, D.; Xie, G.; Huang, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Yang, Q. <i>et al.</i>","Englishabstract":"Aims</h3>                        <p>Acute hepatopancreatic necrosis disease (AHPND) caused by <i>Vibrio parahaemolyticus</i> has emerged as a severe bacterial disease of cultured shrimp. To identify the key virulence factors, two AHPND-causing <i>V. parahaemolyticus</i> (<i>Vp</i><sub>AHPND</sub>) strains (123 and 137) and two non-<i>Vp</i><sub>AHPND</sub> strains (HZ56 and ATCC 17082) were selected.            </p>                     </section>                  <section class=\"article-section__content\" id=\"jam14922-sec-0002\">                        <h3 class=\"article-section__sub-title section1\" id=\"jam14922-sec-0002-title\"> Methods and Results</h3>                        <p>Challenge tests showed that the four strains exhibited different virulence towards shrimp with cumulative mortalities at 48 h postinfection (hpi) ranging from 10 to 92%. The expression of <i>pirAB<sup>VP</sup></i> in strain 123 and 137 was not significantly different. Genomic analysis revealed that the two <i>Vp</i><sub>AHPND</sub> strains contain a plasmid with the PirAB<sup>VP</sup> toxins (<i>pirAB<sup>VP</sup></i>) flanked by the insertion sequence (IS<i>Val1</i>) that has been identified in various locations of chromosomes in <i>Vp</i><sub>AHPND</sub> strains. The two <i>Vp</i><sub>AHPND</sub> strains possessed almost identical virulence factors, while IS<i>Val1</i> disrupted three genes related to flagellar motility in strain 137. Phenotype assay showed that strain 123 possessed the highest growth rate and swimming motility, followed by strain 137, suggesting that the disruption of essential genes mediated by IS<i>Val1</i> significantly affected the virulence level. Transcriptome analysis of two <i>Vp</i><sub>AHPND</sub> strains (123 and 137) further suggested that virulence genes related to the capsule, flagella and primary metabolism were highly expressed in strain 123.            </p>                     </section>                  <section class=\"article-section__content\" id=\"jam14922-sec-0003\">                        <h3 class=\"article-section__sub-title section1\" id=\"jam14922-sec-0003-title\"> Conclusions</h3>                        <p>Here for the first time, it is demonstrated that the virulence of <i>Vp</i><sub>AHPND</sub> is not only determined by the expression of <i>pirAB<sup>VP</sup></i>, but also is mediated by IS<i>Val1</i> which affects the genes involved in flagellar motility and primary metabolism.            </p>                     </section>                  <section class=\"article-section__content\" id=\"jam14922-sec-0004\">                        <h3 class=\"article-section__sub-title section1\" id=\"jam14922-sec-0004-title\"> Significance and Impact of the Study</h3>                        <p>The genomic and transcriptomic analysis of <i>Vp</i><sub>AHPND</sub> strains provides valuable information on the virulence factors affecting the pathogenicity of <i>Vp</i><sub>AHPND.</sub>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Coordination of primary metabolism and virulence factors expression mediates the virulence of <i>Vibrio parahaemolyticus</i> towards cultured shrimp (<i>Penaeus vannamei</i>)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:32:38.900475","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"AHPND; differentially expressed genes; insertion sequence; Vibrio parahaemolyticus; virulence factor","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-06-29","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000599448800001","VABBcode":null,"OpenAcc":0,"Handle":"10.1111/jam.14922"},"refs":null,"anarec":{"AnaID":337472,"PubliDate":2021,"Pagination":"50-67","XtraPublOfAnaID":null,"ISBN":null,"Volume":"131","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45067,"SerRR":"Journal of Applied Microbiology. 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