{"refrec":{"BRefID":33763,"RR":"<b>Tzovenis, I.; De Pauw, N.; Sorgeloos, P.</b> (2003). Optimisation of T-ISO biomass production rich in essential fatty acids: 2. Effect of different light regimes on the production of fatty acids. <i>Aquaculture 216(1-4)</i>: 223-242. <a href=\"http://dx.doi.org/10.1016/S0044-8486(02)00375-7\" target=\"_blank\">dx.doi.org/10.1016/S0044-8486(02)00375-7</a>","BEntID":33763,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquaculture 216(1-4)</i>: 223-242. <a href=\"https://dx.doi.org/10.1016/S0044-8486(02)00375-7\" target=\"_blank\">https://dx.doi.org/10.1016/S0044-8486(02)00375-7</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tzovenis, I.; De Pauw, N.; Sorgeloos, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tzovenis, I. <i>et al.</i>","Englishabstract":"It is well documented that culture conditions affect the fatty acid content of microalgae. We report in this study the fatty acid profiles and <i>n</i> - 3 HUFA productivity of T-ISO, a popular haptophyte in the aquaculture industry, cultured under three photoperiods (24:0, 16:08 and 12:12 h L:D) combined with three photon flux densities (PFD: 120, 220 and 460 µmol photon m<sup>-2</sup> s<sup>-1</sup>), at 25 °C. Sampling took place in both the exponential and post-exponential (light-limited) phase. In general, fatty acid proportions were effected by a strong interaction of L:D× PFD resulting in metabolic changes difficult to be modelled. At the 12:12 and 24:0 h L:D the fatty acid pattern can be summarised as PUFA>SAFA>MUFA, while at 16:08 h L:D as SAFA>PUFA>MUFA reflecting a differential acclimation of the strain under light-dark cycles. At the 12:12 h L:D the PUFA content of biomass was significantly higher than at the other photocycles. PUFA content differences were located in the <i>n</i> - 3 fraction with the <i>n</i> - 6 content being rather constant. The <i>n</i> - 3/<i>n</i> - 6 and DHA/EPA ratios under 24:0 h or 12:12 h L:D were optimal according to the literature for fish and shellfish nutrition requirements. In contrast, the 16:08 h L:D regimes, especially at low PFD, produced inadequate ratios. The production of <i>n</i> - 3 HUFA in T-ISO is essentially influenced by the total photon flux available per day in a similar manner with growth. The capacity of the strain for storing lipid is limited under the conditions tested; consequently, the fatty acid content follows the biomass yield and productivity pattern. Hence, in the context of aquaculture a light regime of 12:12 h L:D and a PFD within the photolimitation-photoinhibition range offers advantages for the culture of T-ISO. If the high investment could be substantiated, continuous cultures under 24:0 h L:D at the same PFD range could serve as an optimisation basis using advanced photobioreactors.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Optimisation of T-ISO biomass production rich in essential fatty acids: 2. Effect of different light regimes on the production of fatty acids","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"T-ISO; photoperiod; fatty acids; photon flux density","OtherDescriptors":"T-ISO; Photoperiod; Fatty acids; Photon flux density","Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2012-03-05","DateCreate":"2003-04-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000180140800020","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/S0044-8486(02)00375-7"},"refs":null,"anarec":{"AnaID":33763,"PubliDate":2003,"Pagination":"223-242","XtraPublOfAnaID":null,"ISBN":null,"Volume":"216","Issue":"1-4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo.  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