{"refrec":{"BRefID":33767,"RR":"<b>Nevejan, N.; Saez, I.; Gajardo, G.; Sorgeloos, P.</b> (2003). Supplementation of EPA and DHA emulsions to a <i>Dunaliella tertiolecta</i> diet: effect on growth and lipid composition of scallop larvae, <i>Argopecten purpuratus</i> (Lamarck, 1819). <i>Aquaculture 217(1-4)</i>: 613-632. <a href=\"http://dx.doi.org/10.1016/S0044-8486(02)00585-9\" target=\"_blank\">dx.doi.org/10.1016/S0044-8486(02)00585-9</a>","BEntID":33767,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquaculture 217(1-4)</i>: 613-632. <a href=\"https://dx.doi.org/10.1016/S0044-8486(02)00585-9\" target=\"_blank\">https://dx.doi.org/10.1016/S0044-8486(02)00585-9</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Nevejan, N.; Saez, I.; Gajardo, G.; Sorgeloos, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Nevejan, N. <i>et al.</i>","Englishabstract":"Hatchery-reared <i>Argopecten purpuratus</i> larvae were fed a <i>Dunaliella tertiolecta</i>-diet (DUN-diet) supplemented with 0%, 20% or 40% of a highly unsaturated fatty acid (HUFA) lipid emulsion (ICES Reference Emulsions), rich in docosahexaenoic acid (22:6<i>n</i> - 3, DHA) and eicosapentaenoic acid (20:5<i>n</i> - 3, EPA). A standard diet (ST-diet), consisting of a mixture of <i>Isochrysis galbana</i>(T-iso) and <i>Chaetoceros neogracile</i> was used as a positive control. The supplementation of 20% and 40% HUFA-emulsion to the DUN-diet led to a significantly better daily growth rate (136-177% as compared to DUN-diet) and a higher percentage of eyed-pediveliger larvae in comparison with the non-supplemented DUN-diet. Practically no eyed-larvae were present when fed with <i>D. tertiolecta</i>only, whereas 17% of the larvae developed an eyespot when fed a 20% HUFA-supplement. An increase of the lipid supplement from 20% to 40% further improved the larval performance, although the difference was not significant and less than compared with the changes between the non-supplemented DUN-diet and the DUN + 20%-diet. The poor performance of larvae that received <i>D. tertiolecta</i> only was attributed to the depletion of the lipid reserves in the triglyceride fraction and possibly to the very low levels of saturated fatty acids 14:0 and 16:0, rather than to the low levels of EPA and DHA (in absolute terms). Limited biosynthesis of DHA from EPA as a precursor molecule is proposed. Despite similar relative and absolute fatty acid levels of DHA and EPA in larvae that received the ST-diet and the DUN + 40%HUFA-diet, the former performed significantly better. Other factors such as the arachidonic acid (20:4<i>n</i> - 6) content and the <i>n</i> - 3/<i>n</i> - 6 ratio are suggested to play a determining role.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Supplementation of EPA and DHA emulsions to a <i>Dunaliella tertiolecta</i> diet: effect on growth and lipid composition of scallop larvae, <i>Argopecten purpuratus</i> (Lamarck, 1819)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"scallop larvae; Argopecten puipuratus; Dunaliella tertiolecta; HUFA; saturated fatty acids; arachidonic acid","OtherDescriptors":"Scallop larvae;  HUFA; Saturated fatty acids; Arachidonic acid","Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2012-04-02","DateCreate":"2003-04-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000181129300046","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/S0044-8486(02)00585-9"},"refs":null,"anarec":{"AnaID":33767,"PubliDate":2003,"Pagination":"613-632","XtraPublOfAnaID":null,"ISBN":null,"Volume":"217","Issue":"1-4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. 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