{"refrec":{"BRefID":337895,"RR":"<b>Blommaert, L.; Vancaester, E.; Huysman, M.J.J.; Osuna-Cruz, C.M.; D'hondt, S.; Lavaud, J.; Lepetit, B.; Winge, P.; Bones, A.M.; Vandepoele, K.; Vyverman, W.; Sabbe, K.</b> (2020). Light regulation of <i>LHCX</i> genes in the benthic diatom <i>Seminavis robusta</i>. <i>Front. Mar. Sci. 7</i>: 192. <a href=\"https://hdl.handle.net/10.3389/fmars.2020.00192\" target=\"_blank\">https://hdl.handle.net/10.3389/fmars.2020.00192</a>","BEntID":334519,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Front. Mar. Sci. 7</i>: 192. <a href=\"https://hdl.handle.net/10.3389/fmars.2020.00192\" target=\"_blank\">https://hdl.handle.net/10.3389/fmars.2020.00192</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Blommaert, L.; Vancaester, E.; Huysman, M.J.J.; Osuna-Cruz, C.M.; D'hondt, S.; Lavaud, J.; Lepetit, B.; Winge, P.; Bones, A.M.; Vandepoele, K.; Vyverman, W.; Sabbe, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Blommaert, L. <i>et al.</i>","Englishabstract":"Intertidal benthic diatoms experience a highly variable light regime, which especially challenges these organisms to cope with excess light energy during low tide. Non-photochemical quenching of chlorophyll fluorescence (NPQ) is one of the most rapid mechanisms diatoms possess to dissipate excess energy. Its capacity is mainly defined by the xanthophyll cycle (XC) and Light-Harvesting Complex X (LHCX) proteins. Whereas the XC and its relation to NPQ have been relatively well-studied in both planktonic and benthic diatoms, our current knowledge about LHCX proteins and their potential involvement in NPQ regulation is largely restricted to planktonic diatoms. While recent studies using immuno-blotting have revealed the presence of light regulated LHCX proteins in benthic diatom communities and isolates, nothing is as yet known about the diversity, identity and transcriptional regulation or function of these proteins. We identified <i>LHCX</i> genes in the draft genome of the model benthic diatom <i>Seminavis robusta</i> and followed their transcriptional regulation during a day/night cycle and during exposure to high light conditions. The <i>S. robusta</i> genome contains 17 LHCX sequences, which is much more than in the sequenced planktonic model diatoms (4–5), but similar to the number of <i>LHCX</i> genes in the sea ice associated diatom <i>Fragilariopsis cylindrus</i>. <i>LHCX</i> diversification in both species, however, appears to have occurred independently. Interestingly, the <i>S. robusta</i> genome contains <i>LHCX</i> genes that are related to the LHCX6 of the model centric diatom <i>Thalassiosira pseudonana</i>, which are lacking in the well-studied pennate model diatom <i>Phaeodactylum tricornutum</i>. All investigated <i>LHCX</i> genes, with exception of <i>SrLHCX6</i>, were upregulated during the daily dark-light transition. Exposure to 2,000 μmol photons m<sup>–2</sup> s<sup>–1</sup>, furthermore, increased transcription of all investigated <i>LHCX</i> genes. Our data suggest that the diversification and involvement of several light regulated <i>LHCX</i> genes in the photophysiology of <i>S. robusta</i> may represent an adaptation to the complex and highly variable light environment this benthic diatom species can be exposed to.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Light regulation of <i>LHCX</i> genes in the benthic diatom <i>Seminavis robusta</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:32:39.259879","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"diatom; microphytobenthos; light stress; LHCX; physiology","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2021-05-30","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000525470500001","VABBcode":null,"OpenAcc":1,"Handle":"10.3389/fmars.2020.00192"},"refs":null,"anarec":{"AnaID":337895,"PubliDate":2020,"Pagination":"192","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":233602,"SerRR":"Frontiers in Marine Science. 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