{"refrec":{"BRefID":338022,"RR":"<b>Tran, P.T.N.; Kumar, V.; Bossier, P.</b> (2020). Do acute hepatopancreatic necrosis disease-causing PirAB<sup>VP</sup> toxins aggravate vibriosis? <i>Emerging Microbes & Infections 9(1)</i>: 1919-1932. <a href=\"https://hdl.handle.net/10.1080/22221751.2020.1811778\" target=\"_blank\">https://hdl.handle.net/10.1080/22221751.2020.1811778</a>","BEntID":334646,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":" <i>Emerging Microbes & Infections 9(1)</i>: 1919-1932. <a href=\"https://hdl.handle.net/10.1080/22221751.2020.1811778\" target=\"_blank\">https://hdl.handle.net/10.1080/22221751.2020.1811778</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tran, P.T.N.; Kumar, V.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tran, P.T.N.; Kumar, V.; Bossier, P.","Englishabstract":"Gram-negative marine bacterium <i>Vibrio parahaemolyticus</i> is an important aquatic pathogen and has been demonstrated to be the causative agent of acute hepatopancreatic necrotic disease (AHPND) in shrimp aquaculture. The AHPND-causing <i>V. parahaemolyticus</i> strains contain a pVA1 plasmid encoding the binary PirA<sup>VP</sup> and PirB<sup>VP</sup> toxins, are the primary virulence factor that mediates AHPND and mortality in shrimp. Since PirAB<sup>VP</sup> toxins are secreted extracellularly, one can hypothesize that PirAB<sup>VP</sup> toxins would aggravate vibriosis in the aquatic environment. To address this, <i>in vivo</i> and <i>in vitro</i> experiments were conducted. Germ-free <i>Artemia franciscana</i> were co-challenged with PirAB<sup>VP</sup> toxins and 10 <i>Vibrio</i> spp. The <i>in vivo</i> results showed that PirAB<sup>VP</sup> toxin interact synergistically with MM30 (a quorum sensing AI-2 deficient mutant) and <i>V. alginolyticus</i> AQ13-91, aggravating vibriosis. However, co-challenge by PirAB<sup>VP</sup> toxins and <i>V. campbellii</i> LMG21363, <i>V. parahaemolyticus</i> CAIM170, <i>V. proteolyticus</i> LMG10942, and <i>V. anguillarum</i> NB10 worked antagonistically, increasing the survival of <i>Artemia</i> larvae. The <i>in vitro</i> results showed that the addition of PirAB<sup>VP</sup> toxins significantly modulated the production of the virulence factors of studied <i>Vibrio</i> spp. Yet these <i>in vitro</i> results did not help to explain the <i>in vivo</i> results. Hence it appears that PirAB<sup>VP</sup> toxins can aggravate vibriosis. However, the dynamics of interaction is strain dependent.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Do acute hepatopancreatic necrosis disease-causing PirAB<sup>VP</sup> toxins aggravate vibriosis?","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"PirAB(VP)toxin; vibriosis; Artemia franciscana; synergistic effect; antagonistic effect","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2022-08-04","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000567083200001","VABBcode":null,"OpenAcc":1,"Handle":"10.1080/22221751.2020.1811778"},"refs":null,"anarec":{"AnaID":338022,"PubliDate":2020,"Pagination":"1919-1932","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":267414,"SerRR":"Emerging Microbes & Infections. 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