{"refrec":{"BRefID":338090,"RR":"<b>Lenaerts, C.; Wells, M.; Hambye, S.; Blankert, B.</b> (2019). Marinobufagenin extraction from <i>Rhinella marina</i> toad glands: alternative approaches for a systematized strategy. <i>Journal of separation science 42(7)</i>: 1384-1392. <a href=\"https://hdl.handle.net/10.1002/jssc.201800879\" target=\"_blank\">https://hdl.handle.net/10.1002/jssc.201800879</a>","BEntID":334714,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Journal of separation science 42(7)</i>: 1384-1392. <a href=\"https://hdl.handle.net/10.1002/jssc.201800879\" target=\"_blank\">https://hdl.handle.net/10.1002/jssc.201800879</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lenaerts, C.; Wells, M.; Hambye, S.; Blankert, B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lenaerts, C. <i>et al.</i>","Englishabstract":"Marinobufagenin is a bufadienolide compound detected mainly in skin and parotoid gland secretions of <i>Rhinella marina</i> (L.) toad. Bufadienolides regulate the Na<sup>+</sup>/K<sup>+</sup>-ATPase pump by inhibiting the cardiotonic steroid dependent-site and act as cardiac inotropes with vasoconstrictive properties. Marinobufagenin and other bufadienolides, such as telocinobufagin and bufalin, are thought to be found endogenously in mammals in salt-sensitive hypertensive states such as essential hypertension, congestive heart-failure, and preeclampsia. The role of marinobufagenin as antimicrobial agent and its cytotoxic potential have also been recognized. The particular interest around marinobufagenin prompts us to consider the <i>Rhinella marina</i> toad venom as a possible source for molecules with pharmacological and/or diagnostic potential. In this article, two different approaches of extraction and purification of marinobufagenin from <i>Rhinella marina</i> (L.) venom are studied: (i) Preparative thin-layer chromatography combined to mass spectrometry and/or ultraviolet detection and (ii) solid-phase extraction coupled with fractionation on high-performance liquid chromatography. Different chromatographic conditions are tested for each approach. The solid-phase extraction combined with high-performance liquid chromatography fractionation approach was preferred as it offered a greater yield, was less time-consuming and allowed us to selectively isolate marinobufagenin. Both protocols aim to provide efficient and convenient methods for toad venom extraction, based on an easily automatable and systematized strategy.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Marinobufagenin extraction from <i>Rhinella marina</i> toad glands: alternative approaches for a systematized strategy","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"high-performance liquid chromatography; marinobufagenin; thin-layer chromatography; toad venom","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2021-05-25","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000466423100009","VABBcode":null,"OpenAcc":0,"Handle":"10.1002/jssc.201800879"},"refs":null,"anarec":{"AnaID":338090,"PubliDate":2019,"Pagination":"1384-1392","XtraPublOfAnaID":null,"ISBN":null,"Volume":"42","Issue":"7","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":236415,"SerRR":"Journal of separation science. 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