{"refrec":{"BRefID":338229,"RR":"<b>Simoens, C.; Korst, A.E.C.; De Pooter, C.M.J.; Lambrechts, H.A.J.; Pattyn, G.G.O.; Faircloth, G.T.; Lardon, F.; Vermorken, J.B.</b> (2003). <i>In vitro</i> interaction between Ecteinascidin 743 (ET-743) and radiation, in relation to its cell cycle effects. <i>British Journal of Cancer 89(12)</i>: 2305-2311. <a href=\"https://hdl.handle.net/10.1038/sj.bjc.6601431\" target=\"_blank\">https://hdl.handle.net/10.1038/sj.bjc.6601431</a>","BEntID":334855,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>British Journal of Cancer 89(12)</i>: 2305-2311. <a href=\"https://hdl.handle.net/10.1038/sj.bjc.6601431\" target=\"_blank\">https://hdl.handle.net/10.1038/sj.bjc.6601431</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Simoens, C.; Korst, A.E.C.; De Pooter, C.M.J.; Lambrechts, H.A.J.; Pattyn, G.G.O.; Faircloth, G.T.; Lardon, F.; Vermorken, J.B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Simoens, C. <i>et al.</i>","Englishabstract":"Ecteinascidin 743 (ET-743) is a new marine-derived agent with promising activity against a number of solid tumours. In four human tumour cell lines, the interaction between ET-743 and radiation was investigated in relation to the effects of ET-743 on the cell cycle, in vitro. Cell survival was measured based on quantitative staining of cellular protein by sulforhodamine B. A 24 h treatment with ET-743 before radiation resulted in a moderate increase in radiosensitivity in three out of four cell lines. Dose enhancement factors greater than or equal to1.8 were observed for concentrations resulting in 52, 46 and 30% cell kill in ECV304, H292 and CAL-27, respectively, whereas in A549 no radiosensitisation was observed (no significant increase in radiosensitivity). According to the combination index analysis, synergism was observed only in ECV304 and CAL-27 cells. A 24 h incubation with ET-743 resulted in a concentration-dependent G2/M block, which might explain the moderate radiosensitising effects in ECV304 and H292. The lack of radiosensitisation in A549 might be due to the S phase delay preceding the G2/M block at the moment of radiation, which only occurred in this cell line. In conclusion, ET-743 has moderate cell line-dependent radiosensitising properties; however, only when cytotoxic concentrations of ET-743 are used. In one of the four cell lines tested, no radiosensitisation was observed.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"<i>In vitro</i> interaction between Ecteinascidin 743 (ET-743) and radiation, in relation to its cell cycle effects","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"ET-743; radiation; radiosensitisation; cell cycle","OtherDescriptors":null,"Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2021-05-31","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000188093600023","VABBcode":null,"OpenAcc":1,"Handle":"10.1038/sj.bjc.6601431"},"refs":null,"anarec":{"AnaID":338229,"PubliDate":2003,"Pagination":"2305-2311","XtraPublOfAnaID":null,"ISBN":null,"Volume":"89","Issue":"12","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":264905,"SerRR":"British Journal of Cancer. 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