{"refrec":{"BRefID":338243,"RR":"<b>Wilmotte, A.; Neefs, J.-M.; De Wachter, R.</b> (1994). Evolutionary affiliation of the marine nitrogen-fixing cyanobacterium <i>Trichodesmium</i> sp. strain NIBB 1067, derived by 16S ribosomal RNA sequence analysis. <i>Microbiology 140(8)</i>: 2159-2164. <a href=\"https://hdl.handle.net/10.1099/13500872-140-8-2159\" target=\"_blank\">https://hdl.handle.net/10.1099/13500872-140-8-2159</a>","BEntID":334869,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Microbiology 140(8)</i>: 2159-2164. <a href=\"https://hdl.handle.net/10.1099/13500872-140-8-2159\" target=\"_blank\">https://hdl.handle.net/10.1099/13500872-140-8-2159</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Wilmotte, A.; Neefs, J.-M.; De Wachter, R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Wilmotte, A.; Neefs, J.-M.; De Wachter, R.","Englishabstract":"The 16S rRNA sequence of <span class=\"jp-italic\">Trichodesmium</span> sp. strain NIBB 1067 was determined and used for the construction of a distance tree and bootstrap analysis. The tree shows that, among the available cyanobacterial 16S rRNA sequences, <span class=\"jp-italic\">Trichodesmium</span> NIBB 1067 has <span class=\"jp-italic\">Oscillatoria</span> PCC 7515 as its closest relative, presenting 94.9% of sequence similarity with the latter strain. This is in contrast to a difference of 9 mol% G + C in mean genomic DNA base composition between the two organisms. Nevertheless, the genotypic heterogeneity presented by a number of strains assigned to the genus <span class=\"jp-italic\">Oscillatoria</span> hinders a taxonomic decision on the separate existence of the genera <span class=\"jp-italic\">Trichodesmium</span> and <span class=\"jp-italic\">Oscillatoria.</span> The sequence of the internal transcribed spacer (ITS) between the 16S and 23S rRNA genes was also determined, as a possible marker to study inter- and intraspecific variability. The ITS contains the genes coding for tRNA<sup xmlns:f=\"http://www.example.org/functions\">lle</sup> and tRNA<sup xmlns:f=\"http://www.example.org/functions\">Ala</sup> and its total length is 547 nucleotides. In six out of eight sequenced clones, there is a duplication of 29 nucleotides, surrounding the 5′ end of the tRNA<sup xmlns:f=\"http://www.example.org/functions\">lle</sup>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Evolutionary affiliation of the marine nitrogen-fixing cyanobacterium <i>Trichodesmium</i> sp. strain NIBB 1067, derived by 16S ribosomal RNA sequence analysis","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Trichodesmium , 16S rRNA sequence analysis, taxonomy, cyanobacteria, internal transcribed spacer","OtherDescriptors":null,"Notes":null,"AnaPub":1994,"MonPub":null,"DateUpdate":"2021-06-01","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1994PC69300042","VABBcode":null,"OpenAcc":1,"Handle":"10.1099/13500872-140-8-2159"},"refs":null,"anarec":{"AnaID":338243,"PubliDate":1994,"Pagination":"2159-2164","XtraPublOfAnaID":null,"ISBN":null,"Volume":"140","Issue":"8","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43402,"SerRR":"Microbiology. 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