{"refrec":{"BRefID":338244,"RR":"<b>Malissard, M.; Duez, C.; Guinand, M.; Vacheron, M.-J.; Michel, G.; Marty, N.; Joris, B.; Thamm, I.; Ghuysen, J.-M.</b> (1993). Sequence of a gene encoding a (poly ManA) alginate lyase active on <i>Pseudomonas aeruginosa</i> alginate. <i>FEMS Microbiol. Lett. 110(1)</i>: 101-106. <a href=\"https://hdl.handle.net/10.1111/j.1574-6968.1993.tb06302.x\" target=\"_blank\">https://hdl.handle.net/10.1111/j.1574-6968.1993.tb06302.x</a>","BEntID":334870,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>FEMS Microbiol. Lett. 110(1)</i>: 101-106. <a href=\"https://hdl.handle.net/10.1111/j.1574-6968.1993.tb06302.x\" target=\"_blank\">https://hdl.handle.net/10.1111/j.1574-6968.1993.tb06302.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Malissard, M.; Duez, C.; Guinand, M.; Vacheron, M.-J.; Michel, G.; Marty, N.; Joris, B.; Thamm, I.; Ghuysen, J.-M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Malissard, M. <i>et al.</i>","Englishabstract":"The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originated from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870–1872). The alginate lyase produced by <em>Escherichia coli</em> TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a <em>Pseudomonas aeruginosa</em> alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385–8391).","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Sequence of a gene encoding a (poly ManA) alginate lyase active on <i>Pseudomonas aeruginosa</i> alginate","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:32:23.490537","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"(poly ManA) Alginate lyase, Gene cloning, Primary structure, Pseudomonas aeruginosa","OtherDescriptors":null,"Notes":null,"AnaPub":1993,"MonPub":null,"DateUpdate":"2021-06-01","DateCreate":"2021-05-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1993LG93600017","VABBcode":null,"OpenAcc":0,"Handle":"10.1111/j.1574-6968.1993.tb06302.x"},"refs":null,"anarec":{"AnaID":338244,"PubliDate":1993,"Pagination":"101-106","XtraPublOfAnaID":null,"ISBN":null,"Volume":"110","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42868,"SerRR":"FEMS. 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