{"refrec":{"BRefID":339170,"RR":"<b>Korlevic, M.; Markovski, M.; Zhao, Z.; Herndl, G.; Najdek, M.</b> (2021). Selective DNA and protein isolation from marine macrophyte surfaces. <i>Front. Microbiol. 12</i>: 665999. <a href=\"https://dx.doi.org/10.3389/fmicb.2021.665999\" target=\"_blank\">https://dx.doi.org/10.3389/fmicb.2021.665999</a>","BEntID":335805,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Front. Microbiol. 12</i>: 665999. <a href=\"https://dx.doi.org/10.3389/fmicb.2021.665999\" target=\"_blank\">https://dx.doi.org/10.3389/fmicb.2021.665999</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Korlevic, M.; Markovski, M.; Zhao, Z.; Herndl, G.; Najdek, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Korlevic, M. <i>et al.</i>","Englishabstract":"Studies of unculturable microbes often combine methods, such as 16S rRNA    sequencing, metagenomics, and metaproteomics. To apply these techniques to    the microbial community inhabiting the surfaces of marine macrophytes, it    is advisable to perform a selective DNA and protein isolation prior to the    analysis to avoid biases due to the host material being present in high    quantities. Two protocols for DNA and protein isolation were adapted for    selective extractions of DNA and proteins from epiphytic communitiesinhabiting the surfaces of two marine macrophytes, the seagrass    <i>Cymodocea nodosa</i> and the macroalga <i>Caulerpa cylindracea</i>.    Protocols showed an almost complete removal of the epiphytic community    regardless of the sampling season, station, settlement, or host species.    The obtained DNA was suitable for metagenomic and 16S rRNA sequencing,    while isolated proteins could be identified by mass spectrometry. Low    presence of host DNA and proteins in the samples indicated a high    specificity of the protocols. The procedures are based on universally    available laboratory chemicals making the protocols widely applicable.    Taken together, the adapted protocols ensure an almost complete removal of    the macrophyte epiphytic community. The procedures are selective for    microbes inhabiting macrophyte surfaces and provide DNA and proteins    applicable in 16S rRNA sequencing, metagenomics, and metaproteomics.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Selective DNA and protein isolation from marine macrophyte surfaces","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-08 01:31:23.988045","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"selective isolation; DNA; proteins; marine macrophytes; <i>Cymodocea nodosa</i>; <i>Caulerpa cylindracea</i>","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-06-18","DateCreate":"2021-06-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000658309900001","VABBcode":null,"OpenAcc":1,"DOI":"10.3389/fmicb.2021.665999"},"refs":null,"anarec":{"AnaID":339170,"PubliDate":2021,"Pagination":"665999","XtraPublOfAnaID":null,"ISBN":null,"Volume":"12","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":234940,"SerRR":"Frontiers in Microbiology. 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