{"refrec":{"BRefID":339516,"RR":"<b>Velázquez-Sánchez, C.; Vences-Guzmán, M.Á.; Moreno, S.; Tinoco-Valencia, R.; Espín, G.; Guzmán, J.; Sahonero Canavesi, D.X.; Sohlenkamp, C.; Segura, D.</b> (2021). PsrA positively regulates the unsaturated fatty acid synthesis operon <i>fabAB</i> in <i>Azotobacter vinelandii</i>. <i>Microbiological Research 249</i>: 126775. <a href=\"https://dx.doi.org/10.1016/j.micres.2021.126775\" target=\"_blank\">https://dx.doi.org/10.1016/j.micres.2021.126775</a>","BEntID":336151,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Microbiological Research 249</i>: 126775. <a href=\"https://dx.doi.org/10.1016/j.micres.2021.126775\" target=\"_blank\">https://dx.doi.org/10.1016/j.micres.2021.126775</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Velázquez-Sánchez, C.; Vences-Guzmán, M.Á.; Moreno, S.; Tinoco-Valencia, R.; Espín, G.; Guzmán, J.; Sahonero Canavesi, D.X.; Sohlenkamp, C.; Segura, D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Velázquez-Sánchez, C. <i>et al.</i>","Englishabstract":"In <i>Pseudomonas</i> spp. PsrA, a transcriptional activator of the<i>rpoS</i> gene, regulates fatty acid catabolism by repressing the    <i>fadBA5</i> β-oxidation operon. In <i>Azotobacter vinelandii,</i> a soil    bacterium closely related to <i>Pseudomonas</i> species, PsrA is also an    activator of <i>rpoS</i> expression, although its participation in the    regulation of lipid metabolism has not been analyzed. In this work we found    that inactivation of <i>psrA</i> had no effect on the expression of    β-oxidation genes in this bacterium, but instead decreased expression of    the unsaturated fatty acid biosynthetic operon <i>fabAB</i>    (3-hydroxydecanoyl-ACP dehydratase/isomerase and 3-ketoacyl-ACP synthase    I). This inactivation also reduced the unsaturated fatty acid content, as    revealed by the thin-layer chromatographic analysis, and confirmed by gas    chromatography; notably, there was also a lower content of cyclopropane    fatty acids, which are synthesized from unsaturated fatty acids. The    absence of PsrA has no effect on the growth rate, but showed loss of cell    viability during long-term growth, in accordance with the role of these    unsaturated and cyclopropane fatty acids in the protection of membranes.    Finally, an electrophoretic mobility shift assay revealed specific binding    of PsrA to the <i>fabA</i> promoter region, where a putative binding site    for this regulator was located. Taken together, our data show that PsrA    plays an important role in the regulation of unsaturated fatty acids    metabolism in <i>A. vinelandii</i> by positively regulating <i>fabAB</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"PsrA positively regulates the unsaturated fatty acid synthesis operon <i>fabAB</i> in <i>Azotobacter vinelandii</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<i>Azotobacter vinelandii</i>; Transcriptional regulation; Unsaturated fatty acids; Cyclopropane fatty acids","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-08-10","DateCreate":"2021-07-02","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000663761900003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.micres.2021.126775"},"refs":null,"anarec":{"AnaID":339516,"PubliDate":2021,"Pagination":"126775","XtraPublOfAnaID":null,"ISBN":null,"Volume":"249","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":274722,"SerRR":"Microbiological Research: Jena.  ISSN 0944-5013","StandardTitleSer":"Microbiological Research","ISSN":"0944-5013","AbbrevSer":null,"StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Velázquez-Sánchez","Firstname":"Claudia","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":458609,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Vences-Guzmán","Firstname":"Miguel Ángel","Initials":"M.Á.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":458610,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Moreno","Firstname":"Soledad","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":458611,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Tinoco-Valencia","Firstname":"Raunel","Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":458612,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Espín","Firstname":"Guadalupe","Initials":"G.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":458613,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Guzmán","Firstname":"Josefina","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":458614,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sahonero Canavesi","Firstname":"Diana","Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":462757,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0003-1928-5301","PersID":40400,"InsID":13655},{"AutName":"Sohlenkamp","Firstname":"Christian","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":372454,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Segura","Firstname":"Daniel","Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":336151,"AutID":458617,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":336151,"BEntPrivate":null,"availability":[{"BInstID":362869,"LibID":2779,"BRefID":339516,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"362869","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":261,"Classification":"NIOZ files","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":null,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":null,"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Azotobacter vinelandii"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":336151,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":336151,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.micres.2021.126775","externalID":"10.1016/j.micres.2021.126775","URLTypeCode":"DOI","URLID":94666,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Azotobacter vinelandii","AphiaID":null,"TaxtID":50306}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Azotobacter vinelandii","DutchTerm":null,"AphiaID":null,"TaxtID":50306}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"daan.van.loon@nioz.nl","newSesDate":{"date":"2021-07-02 09:24:14.350000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"daan.van.loon@nioz.nl","updSesDate":{"date":"2021-08-10 09:41:05.047000","timezone_type":3,"timezone":"Europe/Brussels"}}}