{"refrec":{"BRefID":339522,"RR":"<b>de Jonge, D.S.W.; Merten, V.; Bayer, T.; Puebla, O.; Reusch, Th.B.H.; Hoving, H.-J.T.</b> (2021). A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region. <i>Royal Society Open Science 8(2)</i>: 201388. <a href=\"https://dx.doi.org/10.1098/rsos.201388\" target=\"_blank\">https://dx.doi.org/10.1098/rsos.201388</a>","BEntID":336157,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Royal Society Open Science 8(2)</i>: 201388. <a href=\"https://dx.doi.org/10.1098/rsos.201388\" target=\"_blank\">https://dx.doi.org/10.1098/rsos.201388</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"de Jonge, D.S.W.; Merten, V.; Bayer, T.; Puebla, O.; Reusch, Th.B.H.; Hoving, H.-J.T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"de Jonge, D.S.W. <i>et al.</i>","Englishabstract":"Cephalopods are pivotal components of marine food webs, but biodiversity studies are hampered by challenges to sample these agile marine molluscs. Metabarcoding of environmental DNA (eDNA) is a potentially powerful technique to study oceanic cephalopod biodiversity and distribution but has not been applied thus far. We present a novel universal primer pair for metabarcoding cephalopods from eDNA, <i>Ceph18S</i> (Forward: 5′-CGC GGC GCT ACA TAT TAG AC-3′, Reverse: 5′-GCA CTT AAC CGA CCG TCG AC-3′). The primer pair targets the hypervariable region V2 of the nuclear 18S rRNA gene and amplifies a relatively short target sequence of approximately 200 bp in order to allow the amplification of degraded DNA. <i>In silico</i> tests on a reference database and empirical tests on DNA extracts from cephalopod tissue estimate that 44–66% of cephalopod species, corresponding to about 310–460 species, can be amplified and identified with this primer pair. A multi-marker approach with the novel <i>Ceph18S</i> and two previously published cephalopod mitochondrial 16S rRNA primer sets targeting the same region (Jarman <i>et al</i>. 2006 <i>Mol. Ecol. Notes.</i><b>6</b>, 268–271; Peters <i>et al</i>. 2015 <i>Mar. Ecol.</i><b>36</b>, 1428–1439) is estimated to amplify and identify 89% of all cephalopod species, of which an estimated 19% can only be identified by <i>Ceph18S</i>. All sequences obtained with <i>Ceph18S</i> were submitted to GenBank, resulting in new 18S rRNA sequences for 13 cephalopod taxa.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Cephalopoda, metabarcoding, environmental DNA, universal primer","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-07-05","DateCreate":"2021-07-02","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000672609500001","VABBcode":null,"OpenAcc":1,"DOI":"10.1098/rsos.201388"},"refs":null,"anarec":{"AnaID":339522,"PubliDate":2021,"Pagination":"201388","XtraPublOfAnaID":null,"ISBN":null,"Volume":"8","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":247720,"SerRR":"Royal Society Open Science. 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