{"refrec":{"BRefID":339679,"RR":"<b>Ribes-Navarro, A.; Navarro, J.C.; Hontoria, F.; Kabeya, N.; Standal, I.B.; Evjemo, J.O.; Monroig, Ó.</b> (2021). Biosynthesis of long-chain polyunsaturated fatty acids in marine gammarids: molecular cloning and functional characterisation of three fatty acyl elongases. <i>Mar. Drugs 19(4)</i>: 226. <a href=\"https://dx.doi.org/10.3390/md19040226\" target=\"_blank\">https://dx.doi.org/10.3390/md19040226</a>","BEntID":336314,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Drugs 19(4)</i>: 226. <a href=\"https://dx.doi.org/10.3390/md19040226\" target=\"_blank\">https://dx.doi.org/10.3390/md19040226</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ribes-Navarro, A.; Navarro, J.C.; Hontoria, F.; Kabeya, N.; Standal, I.B.; Evjemo, J.O.; Monroig, Ó.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ribes-Navarro, A. <i>et al.</i>","Englishabstract":"Long-chain (C<sub>20–24</sub>) polyunsaturated fatty acids (LC-PUFAs) are essential nutrients that are mostly produced in marine ecosystems. Previous studies suggested that gammarids have some capacity to endogenously produce LC-PUFAs. This study aimed to investigate the repertoire and functions of elongation of very long-chain fatty acid (Elovl) proteins in gammarids. Our results show that gammarids have, at least, three distinct <span class=\"html-italic\">elovl</span> genes with putative roles in LC-PUFA biosynthesis. Phylogenetics allowed us to classify two elongases as Elovl4 and Elovl6, as they were bona fide orthologues of vertebrate Elovl4 and Elovl6. Moreover, a third elongase was named as “Elovl1/7-like” since it grouped closely to the Elovl1 and Elovl7 found in vertebrates. Molecular analysis of the deduced protein sequences indicated that the gammarid Elovl4 and Elovl1/7-like were indeed polyunsaturated fatty acid (PUFA) elongases, whereas Elovl6 had molecular features typically found in non-PUFA elongases. This was partly confirmed in the functional assays performed on the marine gammarid <span class=\"html-italic\">Echinogammarus marinus</span> Elovl, which showed that both Elovl4 and Elovl1/7-like elongated PUFA substrates ranging from C<sub>18</sub> to C<sub>22</sub>. <span class=\"html-italic\">E. marinus</span> Elovl6 was only able to elongate C<sub>18</sub> PUFA substrates, suggesting that this enzyme does not play major roles in the LC-PUFA biosynthesis of gammarids.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Biosynthesis of long-chain polyunsaturated fatty acids in marine gammarids: molecular cloning and functional characterisation of three fatty acyl elongases","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-12 01:32:04.843642","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Echinogammarus marinus, elovl enzymes, LC-PUFA biosynthesis, functional characterisation, gammarids","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-07-07","DateCreate":"2021-07-06","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000643247500001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/md19040226"},"refs":null,"anarec":{"AnaID":339679,"PubliDate":2021,"Pagination":"226","XtraPublOfAnaID":null,"ISBN":null,"Volume":"19","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":113252,"SerRR":"Marine Drugs. 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