Document of bibliographic reference 339679

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Biosynthesis of long-chain polyunsaturated fatty acids in marine gammarids: molecular cloning and functional characterisation of three fatty acyl elongases

Abstract

Long-chain (C_20–24) polyunsaturated fatty acids (LC-PUFAs) are essential nutrients that are mostly produced in marine ecosystems. Previous studies suggested that gammarids have some capacity to endogenously produce LC-PUFAs. This study aimed to investigate the repertoire and functions of elongation of very long-chain fatty acid (Elovl) proteins in gammarids. Our results show that gammarids have, at least, three distinct elovl genes with putative roles in LC-PUFA biosynthesis. Phylogenetics allowed us to classify two elongases as Elovl4 and Elovl6, as they were bona fide orthologues of vertebrate Elovl4 and Elovl6. Moreover, a third elongase was named as “Elovl1/7-like” since it grouped closely to the Elovl1 and Elovl7 found in vertebrates. Molecular analysis of the deduced protein sequences indicated that the gammarid Elovl4 and Elovl1/7-like were indeed polyunsaturated fatty acid (PUFA) elongases, whereas Elovl6 had molecular features typically found in non-PUFA elongases. This was partly confirmed in the functional assays performed on the marine gammarid Echinogammarus marinus Elovl, which showed that both Elovl4 and Elovl1/7-like elongated PUFA substrates ranging from C₁₈ to C₂₂. E. marinus Elovl6 was only able to elongate C₁₈ PUFA substrates, suggesting that this enzyme does not play major roles in the LC-PUFA biosynthesis of gammarids.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000643247500001

Bibliographic citation

Ribes-Navarro, A.; Navarro, J.C.; Hontoria, F.; Kabeya, N.; Standal, I.B.; Evjemo, J.O.; Monroig, Ó. (2021). Biosynthesis of long-chain polyunsaturated fatty acids in marine gammarids: molecular cloning and functional characterisation of three fatty acyl elongases. Mar. Drugs 19(4): 226. https://dx.doi.org/10.3390/md19040226

Topic

Marine

Is peer reviewed

true

Access rights

open access

Is accessible for free

true