{"refrec":{"BRefID":339955,"RR":"<b>Ahn, A.-C.; Jongepier, E.; Schuurmans, J.M.; Rijpstra, W.I.C.; Sinninghe Damsté, J.S; Galinski, E.A.; Roman, P.; Sorokin, D.Y.; Muyzer, G.</b> (2021). Molecular and physiological adaptations to low temperature in <i>Thioalkalivibrio</i> strains isolated from soda lakes with different temperature regimes. <i>mSystems 6(2)</i>: e01202-20. <a href=\"https://dx.doi.org/10.1128/msystems.01202-20\" target=\"_blank\">https://dx.doi.org/10.1128/msystems.01202-20</a>","BEntID":336590,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>mSystems 6(2)</i>: e01202-20. <a href=\"https://dx.doi.org/10.1128/msystems.01202-20\" target=\"_blank\">https://dx.doi.org/10.1128/msystems.01202-20</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ahn, A.-C.; Jongepier, E.; Schuurmans, J.M.; Rijpstra, W.I.C.; Sinninghe Damsté, J.S; Galinski, E.A.; Roman, P.; Sorokin, D.Y.; Muyzer, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ahn, A.-C. <i>et al.</i>","Englishabstract":"The genus <i>Thioalkalivibrio</i> comprises sulfur-oxidizing bacteria    thriving in soda lakes at high pH and salinity. Depending on the    geographical location and the season, these lakes can strongly vary in    temperature. To obtain a comprehensive understanding of the molecular and    physiological adaptations to low temperature, we compared the responses of    two <i>Thioalkalivibrio</i> strains to low (10°C) and high (30°C)    temperatures. For this, the strains were grown under controlled conditions    in chemostats and analyzed for their gene expression (RNA sequencing    [RNA-Seq]), membrane lipid composition, and glycine betaine content. The    strain <i>Thioalkalivibrio versutus</i> AL2<sup>T</sup> originated from a    soda lake in southeast Siberia that is exposed to strong seasonaltemperature differences, including freezing winters, whereas    <i>Thioalkalivibrio nitratis</i> ALJ2 was isolated from an East African    Rift Valley soda lake with a constant warm temperature the year round. The    strain AL2<sup>T</sup> grew faster than ALJ2 at 10°C, likely due to its    3-fold-higher concentration of the osmolyte glycine betaine. Moreover,    significant changes in the membrane lipid composition were observed for    both strains, leading to an increase in their unsaturated fatty acid    content via the Fab pathway to avoid membrane stiffness. Genes for the    transcriptional and translational machinery, as well as for counteracting    cold-induced hampering of nucleotides and proteins, were upregulated.    Oxidative stress was reduced by induction of vitamin B<sub>12</sub>    biosynthesis genes, and growth at 10°C provoked downregulation of genes    involved in the second half of the sulfur oxidation pathway. Genes for    intracellular signal transduction were differentially expressed, and    interestingly, AL2<sup>T</sup> upregulated flagellin expression, whereas    ALJ2 downregulated it.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Molecular and physiological adaptations to low temperature in <i>Thioalkalivibrio</i> strains isolated from soda lakes with different temperature regimes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<i>Thioalkalivibrio</i>; soda lake; system biology; RNA-Seq; membrane lipid composition; glycine betaine","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-07-12","DateCreate":"2021-07-11","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000665831100016","VABBcode":null,"OpenAcc":1,"DOI":"10.1128/msystems.01202-20"},"refs":null,"anarec":{"AnaID":339955,"PubliDate":2021,"Pagination":"e01202-20","XtraPublOfAnaID":null,"ISBN":null,"Volume":"6","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":291446,"SerRR":"mSystems. 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