{"refrec":{"BRefID":340006,"RR":"<b>Chalini, K.; Johnson, M.; Almeida, R.S.; Coutinho, H.D.M.</b> (2021). Optimization of DNA isolation and amplification protocol for <i>Gracilaria</i> and <i>Sargassum</i> species of Tamil Nadu coast. <i>Aquat. Bot. 171</i>: 103377. <a href=\"https://dx.doi.org/10.1016/j.aquabot.2021.103377\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquabot.2021.103377</a>","BEntID":336641,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Bot. 171</i>: 103377. <a href=\"https://dx.doi.org/10.1016/j.aquabot.2021.103377\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquabot.2021.103377</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Chalini, K.; Johnson, M.; Almeida, R.S.; Coutinho, H.D.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Chalini, K. <i>et al.</i>","Englishabstract":"The present study proposes a simple and rapid protocol to isolate DNA from marine algae of the genus <i>Sargassum</i> and <i>Gracilaria</i>. To identify the easy, fast and low cost method, four methods of DNA isolation viz., The Robertson Labs methods, STE Buffer method, PureFast-Plant Genomic DNA purification kit by Helini Biomolecules, Chennai and DNA Isolation by Heat Lysis Method were chosen for the present study. To optimize the DNA isolation methods, the thalli of <i>Sargassum plagiophyllum</i> C. Ag. and <i>Gracilaria Salicornia</i> C.Ag. were employed as a source of DNA. The DNA isolated based on three genomic DNA extraction protocols and by heat lysis method varied with reference to method employed for the isolation. By heat lysis method, the DNA of <i>S. plagiophyllum</i> and <i>G. salicornia</i> was isolated and simultaneously amplified (400 bp) using 23S rRNA gene primers. With these results, the DNA of <i>G. salicornia</i>, <i>G. edulis</i>, <i>G. corticata</i>, <i>G. fergusonii</i>, <i>G. verrucosa</i>, <i>S. aquifolium</i>, <i>S. plagiophyllum</i>, <i>S. polycystum</i>, <i>S. tenerrimum</i> and <i>S. swartzii</i> were isolated and amplified using heat lysis method. The heat lysis method is thereby proven to be efficient, rapid, simple, cost effective and requires only a small fraction of the sample. 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