{"refrec":{"BRefID":341318,"RR":"<b>Badis, Y.; Scornet, D.; Harada, M.; Caillard, C.; Godfroy, O.; Raphalen, M.; Gachon, C.M.M.; Coelho, S.M.; Motomura, T.; Nagasato, C.; Cock, J.M.</b> (2021). Targeted CRISPR‐Cas9‐based gene knockouts in the model brown alga <i>Ectocarpus</i>. <i>New Phytol. 231(5)</i>: 2077-2091. <a href=\"https://dx.doi.org/10.1111/nph.17525\" target=\"_blank\">https://dx.doi.org/10.1111/nph.17525</a>","BEntID":337953,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>New Phytol. 231(5)</i>: 2077-2091. <a href=\"https://dx.doi.org/10.1111/nph.17525\" target=\"_blank\">https://dx.doi.org/10.1111/nph.17525</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Badis, Y.; Scornet, D.; Harada, M.; Caillard, C.; Godfroy, O.; Raphalen, M.; Gachon, C.M.M.; Coelho, S.M.; Motomura, T.; Nagasato, C.; Cock, J.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Badis, Y. <i>et al.</i>","Englishabstract":"Brown algae are an important group of multicellular eukaryotes, phylogenetically distinct from both the animal and land plant lineages. <i>Ectocarpus</i> has emerged as a model organism to study diverse aspects of brown algal biology, but this system currently lacks an effective reverse genetics methodology to analyse the functions of selected target genes.            </li>                                    <li>Here, we report that mutations at specific target sites are generated following the introduction of CRISPR-Cas9 ribonucleoproteins into <i>Ectocarpus</i> cells, using either biolistics or microinjection as the delivery method.            </li>                                    <li>Individuals with mutations affecting the <i>ADENINE PHOSPHORIBOSYL TRANSFERASE</i> (<i>APT</i>) gene were isolated following treatment with 2-fluoroadenine, and this selection system was used to isolate individuals in which mutations had been introduced simultaneously at <i>APT</i> and at a second gene. This double mutation approach could potentially be used to isolate mutants affecting any <i>Ectocarpus</i> gene, providing an effective reverse genetics tool for this model organism.            </li>                                    <li>The availability of this tool will significantly enhance the utility of <i>Ectocarpus</i> as a model organism for this ecologically and economically important group of marine organisms. Moreover, the methodology described here should be readily transferable to other brown algal species.            </li>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Targeted CRISPR‐Cas9‐based gene knockouts in the model brown alga <i>Ectocarpus</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:31:50.621795","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"brown alga, Cas9, CRISPR, everse genetics, transformation","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2022-09-19","DateCreate":"2021-08-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000671593500001","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/nph.17525"},"refs":null,"anarec":{"AnaID":341318,"PubliDate":2021,"Pagination":"2077-2091","XtraPublOfAnaID":null,"ISBN":null,"Volume":"231","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43570,"SerRR":"New Phytologist. 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