{"refrec":{"BRefID":34253,"RR":"<b>Ohkubo, N.; Matsubara, T.</b> (2002). Sequential utilization of free amino acids, yolk proteins and lipids in developing eggs and yolk-sac larvae of barfin flounder <i>Verasper moseri</i>. <i>Mar. Biol. (Berl.) 140</i>: 187-196. <a href=\"https://dx.doi.org/10.1007/s002270100647\" target=\"_blank\">https://dx.doi.org/10.1007/s002270100647</a>","BEntID":34253,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biol. (Berl.) 140</i>: 187-196. <a href=\"https://dx.doi.org/10.1007/s002270100647\" target=\"_blank\">https://dx.doi.org/10.1007/s002270100647</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ohkubo, N.; Matsubara, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ohkubo, N.; Matsubara, T.","Englishabstract":"Changes in the contents of free amino acids (FAA), M r 170,000 lipovitellin (oLv B), that is the major yolk protein in ovulated eggs, and lipids were measured in developing eggs and yolk-sac larvae of barfin flounder (<i>Verasper moseri</i>) to elucidate the sequential utilization of these nutrient stocks before the .rst feed. Hatching takes place on the 10th day after fertilization at water temperature of 8 C, and the hatched larvae absorb almost all of their yolk masses within 21 days after fertilization. The total FAA content showed no change during the first 4 days,  then decreased to about 13%of the initial level by the 13th day after fertilization. During the consumption of FAA, non-essential amino acids tend to decrease earlier than essential amino acids. The native molecular weight and immunoreactivity of M r 170,000 oLv B did not show any change during the first 16 days after fertilization, though the sodium dodecyl sulphate-polyacrylamide gel electrophoresis patterns of oLv B showed serial change. The oLv B contents, measured by quantitative immunodi fusion using antiserum against oLv B of ovulated eggs, were approximately stable during the 13 days after fertilization, then decreased rapidly until the end of yolk-sac absorption. Phospholipids (PL), which seem mostly to bind with lipovitellin-proteins, decreased gradually after hatching coincident with the decrease in oLv B and M r 330,000 lipoprotein. From these results, we conclude that there are three periods for sequential nutrient utilization in barfin flounder embryos and larvae: (1) pre- FAA utilization period, 0 -4th day; (2) FAA utilization period, 4th-13th day; (3) oLv B and PL utilization period, 16th  -21st day post-fertilization.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Sequential utilization of free amino acids, yolk proteins and lipids in developing eggs and yolk-sac larvae of barfin flounder <i>Verasper moseri</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-24 01:31:01.023162","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2002,"MonPub":null,"DateUpdate":"2003-04-25","DateCreate":"2003-04-25","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000174234500018","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s002270100647"},"refs":null,"anarec":{"AnaID":34253,"PubliDate":2002,"Pagination":"187-196","XtraPublOfAnaID":null,"ISBN":null,"Volume":"140","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43352,"SerRR":"Marine Biology: International Journal on Life in Oceans and Coastal Waters. Springer: Heidelberg; Berlin.  ISSN 0025-3162; e-ISSN 1432-1793","StandardTitleSer":"Marine Biology: International Journal on Life in Oceans and Coastal Waters","ISSN":"0025-3162","AbbrevSer":"Mar. Biol. (Berl.)","StandardTitleMon":null,"StartPage":187,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ohkubo","Firstname":null,"Initials":"N.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":34253,"AutID":6935,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Matsubara","Firstname":null,"Initials":"T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":34253,"AutID":6783,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer","City":"Heidelberg; Berlin"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":34253,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s002270100647","externalID":"10.1007/s002270100647","URLTypeCode":"DOI","URLID":110044,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Milh, Dominique, D.","newSesDate":{"date":"2003-04-25 08:39:55.733000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Milh, Dominique, D.","updSesDate":{"date":"2003-04-25 08:39:55.733000","timezone_type":3,"timezone":"Europe/Brussels"}}}