{"refrec":{"BRefID":344235,"RR":"<b>Baquero, D.P.; Gazi, A.D.; Sachse, M.; Liu, J.; Schmitt, C.; Moya-Nilges, M.; Schouten, S.; Prangishvili, D.; Krupovic, M.</b> (2021). A filamentous archaeal virus is enveloped inside the cell and released through pyramidal portals. <i>Proc. Natl. Acad. Sci. U.S.A. 118(32)</i>: e2105540118. <a href=\"https://dx.doi.org/10.1073/pnas.2105540118\" target=\"_blank\">https://dx.doi.org/10.1073/pnas.2105540118</a>","BEntID":340870,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Proc. Natl. Acad. Sci. U.S.A. 118(32)</i>: e2105540118. <a href=\"https://dx.doi.org/10.1073/pnas.2105540118\" target=\"_blank\">https://dx.doi.org/10.1073/pnas.2105540118</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Baquero, D.P.; Gazi, A.D.; Sachse, M.; Liu, J.; Schmitt, C.; Moya-Nilges, M.; Schouten, S.; Prangishvili, D.; Krupovic, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Baquero, D.P. <i>et al.</i>","Englishabstract":"The majority of viruses infecting hyperthermophilic archaea display unique    virion architectures and are evolutionarily unrelated to viruses of    bacteria and eukaryotes. The lack of relationships to other known viruses    suggests that the mechanisms of virus–host interaction in Archaea are also    likely to be distinct. To gain insights into archaeal virus–host    interactions, we studied the life cycle of the enveloped, ∼2-μm-longSulfolobus islandicus filamentous virus (SIFV), a member of the family    <i>Lipothrixviridae</i> infecting a hyperthermophilic and acidophilic    archaeon <i>Saccharolobus islandicus</i> LAL14/1. Using dual-axis electron    tomography and convolutional neural network analysis, we characterize the    life cycle of SIFV and show that the virions, which are nearly two times    longer than the host cell diameter, are assembled in the cell cytoplasm,    forming twisted virion bundles organized on a nonperfect hexagonal lattice.    Remarkably, our results indicate that envelopment of the helical    nucleocapsids takes place inside the cell rather than by budding as in the    case of most other known enveloped viruses. The mature virions are released    from the cell through large (up to 220 nm in diameter), six-sided pyramidal    portals, which are built from multiple copies of a single    89-amino-acid-long viral protein gp43. The overexpression of this protein    in <i>Escherichia coli</i> leads to pyramid formation in the bacterial    membrane. Collectively, our results provide insights into the assembly and    release of enveloped filamentous viruses and illuminate the evolution of    virus–host interactions in Archaea.</p>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A filamentous archaeal virus is enveloped inside the cell and released through pyramidal portals","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:32:25.037541","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"hyperthermophilic archaea; virus egress; virus assembly; archaeal viruses; cell lysis","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-10-14","DateCreate":"2021-08-27","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000685043800004","VABBcode":null,"OpenAcc":1,"DOI":"10.1073/pnas.2105540118"},"refs":null,"anarec":{"AnaID":344235,"PubliDate":2021,"Pagination":"e2105540118","XtraPublOfAnaID":null,"ISBN":null,"Volume":"118","Issue":"32","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43690,"SerRR":"Proceedings of the National Academy of Sciences of the United States of America. The Academy: Washington, D.C..  ISSN 0027-8424; e-ISSN 1091-6490","StandardTitleSer":"Proceedings of the National Academy of Sciences of the United States of America","ISSN":"0027-8424","AbbrevSer":"Proc. Natl. Acad. Sci. U.S.A.","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Baquero","Firstname":"Diana","Initials":"D.P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":463495,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Gazi","Firstname":"Anastasia","Initials":"A.D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":463496,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sachse","Firstname":"Martin","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":463497,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Liu","Firstname":"Junfeng","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":463498,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Schmitt","Firstname":"Christine","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":463499,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Moya-Nilges","Firstname":"Maryse","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":463500,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Schouten","Firstname":"Stefan","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":210357,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":15342,"InsID":13655},{"AutName":"Prangishvili","Firstname":"David","Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":202767,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Krupovic","Firstname":"Mart","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":340870,"AutID":261886,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":340870,"BEntPrivate":null,"availability":[{"BInstID":368857,"LibID":2779,"BRefID":344235,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"368857","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":262,"Classification":"NIOZ Open Repository - Accepted Manuscripts","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null},{"BInstID":367404,"LibID":2779,"BRefID":344235,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"367404","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":261,"Classification":"NIOZ files","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":null,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"The Academy","City":"Washington, D.C."}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":340870,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":340870,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1073/pnas.2105540118","externalID":"10.1073/pnas.2105540118","URLTypeCode":"DOI","URLID":96365,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"daan.van.loon@nioz.nl","newSesDate":{"date":"2021-08-27 15:42:51.700000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Leonne.van.der.Weegen@nioz.nl","updSesDate":{"date":"2021-10-14 09:16:49.833000","timezone_type":3,"timezone":"Europe/Brussels"}}}