{"refrec":{"BRefID":34630,"RR":"<b>Svärdh, L.</b> (2003). Tissue sampling from live blue mussels, <i>Mytilus edulis</i>: a field study from the Swedish west coast. <i>J. Sea Res. 49(3)</i>: 221-225. <a href=\"http://dx.doi.org/10.1016/s1385-1101(03)00003-0\" target=\"_blank\">http://dx.doi.org/10.1016/s1385-1101(03)00003-0</a>","BEntID":34630,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>J. Sea Res. 49(3)</i>: 221-225. <a href=\"https://dx.doi.org/10.1016/s1385-1101(03)00003-0\" target=\"_blank\">https://dx.doi.org/10.1016/s1385-1101(03)00003-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Svärdh, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Svärdh, L.","Englishabstract":"Histological techniques are often used to study environmental effects on mussels, but since these techniques include killing of the individuals, rare or endangered populations cannot be studied using conventional tissue sampling. This study is an attempt to find a method that can be used repeatedly with the same mussel individual and which does not affect growth and survival. From 200 mussels, <i>Mytilus edulis</i>, tissue was sampled in different ways, such as drilling a hole in the shell or prising apart the shell valves. Two kind of instruments were used, an injection needle and surgery forceps. Some of the drilled mussels had their holes sealed again with cement. Drilling a hole in the shell, removing tissue sample with surgical forceps and then leaving the holes open did not seriously harm the mussels during the two months the experiment lasted. But if the holes were sealed with cement, both length and weight growth were negatively affected (35% lower length growth and 36% lower weight growth compared to the control mussels). Mortality was highest among the drilled and sealed mussels (80% higher than among the other treatments). The vulnerability of the population, the aim of the study and the duration of the experiment should decide what method to use for tissue sampling. For long-term experiments and repeated sampling, opening the mussels by prizing apart the valves is a better alternative than drilling holes in the shells, but depending on the morphology of the species it could be difficult to sample the anterior part of the mussel body. For a short experiment and to sample anterior parts, drilling the shells, leaving the holes open and using surgical forceps, seems to be an acceptable compromise between the different treatments used.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Tissue sampling from live blue mussels, <i>Mytilus edulis</i>: a field study from the Swedish west coast","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-02 08:58:19.190595","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Mytilus edulis; drilling; growth; mortality; sealing; techniques","OtherDescriptors":"Tissue sampling","Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2003-05-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000182443500005","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/s1385-1101(03)00003-0"},"refs":null,"anarec":{"AnaID":34630,"PubliDate":2003,"Pagination":"221-225","XtraPublOfAnaID":null,"ISBN":null,"Volume":"49","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43261,"SerRR":"Journal of Sea Research. Elsevier/Netherlands Institute for Sea Research: Amsterdam; Den Burg.  ISSN 1385-1101; e-ISSN 1873-1414","StandardTitleSer":"Journal of Sea Research","ISSN":"1385-1101","AbbrevSer":"J. Sea Res.","StandardTitleMon":null,"StartPage":221,"Pages":5,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"SE-45296, Strömstad","Email":"lillemor.svardh@marecol.gu.se","Line2":null,"EnvName":"Sweden"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Svärdh","Firstname":"Lillemor","Initials":"L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":34630,"AutID":37094,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":56685,"LibID":36,"BRefID":34630,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"56685","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Amsterdam; Den Burg"},{"PublName":"Netherlands Institute for Sea Research","City":"Amsterdam; Den Burg"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Acceptability","ThestID":39,"Acronym":"ASFA","ThesTermPath":"Acceptability"},{"ThesaurusTerm":"ANE, Sweden, West","ThestID":182209,"Acronym":"ASFA_GEOT","ThesTermPath":"ANE, Sweden, West"},{"ThesaurusTerm":"Environmental effects","ThestID":2807,"Acronym":"ASFA","ThesTermPath":"Environmental effects"},{"ThesaurusTerm":"Methodology","ThestID":5176,"Acronym":"ASFA","ThesTermPath":"Methodology"},{"ThesaurusTerm":"Growth","ThestID":3830,"Acronym":"ASFA","ThesTermPath":"Population functions > Growth"},{"ThesaurusTerm":"Mortality","ThestID":5352,"Acronym":"ASFA","ThesTermPath":"Population functions > Mortality"},{"ThesaurusTerm":"Experimental research","ThestID":2978,"Acronym":"ASFA","ThesTermPath":"Research > Experimental research"},{"ThesaurusTerm":"Testing","ThestID":8468,"Acronym":"ASFA","ThesTermPath":"Testing"}],"thestermsASFA":[{"ThesaurusTerm":"Acceptability"},{"ThesaurusTerm":"ANE, Sweden, West"},{"ThesaurusTerm":"Environmental effects"},{"ThesaurusTerm":"Experimental research"},{"ThesaurusTerm":"Growth"},{"ThesaurusTerm":"Methodology"},{"ThesaurusTerm":"Mortality"},{"ThesaurusTerm":"Testing"}],"taxtermsASFA":[{"TaxTerm":"Mytilus edulis"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":34630,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":34630,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/s1385-1101(03)00003-0","externalID":"10.1016/s1385-1101(03)00003-0","URLTypeCode":"DOI","URLID":1720,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Acceptability","ThestID":39,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"ANE, Sweden, West","ThestID":182209,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Environmental effects","ThestID":2807,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Experimental research","ThestID":2978,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Growth","ThestID":3830,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Methodology","ThestID":5176,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Mortality","ThestID":5352,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Testing","ThestID":8468,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Mytilus edulis","AphiaID":140480,"TaxtID":25121}],"geoterms":null,"othterms":[{"OtherTerm":"Tissue sampling","OthtID":1186}],"asfacodes":[{"Code":3502,"QCode":"Q5"}],"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Acceptability","DutchTerm":null,"ThestID":39,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"ANE, Sweden, West","DutchTerm":null,"ThestID":182209,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Environmental effects","DutchTerm":"Milieueffecten","ThestID":2807,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Experimental research","DutchTerm":"Experimenteel onderzoek","ThestID":2978,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Growth","DutchTerm":"Groei","ThestID":3830,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Methodology","DutchTerm":"methodologie","ThestID":5176,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Mortality","DutchTerm":"Sterfte","ThestID":5352,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Testing","DutchTerm":null,"ThestID":8468,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Mytilus edulis","DutchTerm":"Mossel","AphiaID":140480,"TaxtID":25121}],"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"Tissue sampling","DutchTerm":null,"OthtID":1186}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2003-05-08 15:46:59.290000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2018-05-17 21:48:18.770000","timezone_type":3,"timezone":"Europe/Brussels"}}}