{"refrec":{"BRefID":347291,"RR":"<b>Klunder, L.; van Bleijswijk, J.D.L.; Kleine Schaars, L.; van der Veer, H.W.; Luttikhuizen, P.C.; Bijleveld, A.</b> (2022). Quantification of marine benthic communities with metabarcoding. <i>Mol. Ecol. Resour. 22(3)</i>: 1043-1054. <a href=\"https://dx.doi.org/10.1111/1755-0998.13536\" target=\"_blank\">https://dx.doi.org/10.1111/1755-0998.13536</a>","BEntID":343983,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mol. Ecol. Resour. 22(3)</i>: 1043-1054. <a href=\"https://dx.doi.org/10.1111/1755-0998.13536\" target=\"_blank\">https://dx.doi.org/10.1111/1755-0998.13536</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Klunder, L.; van Bleijswijk, J.D.L.; Kleine Schaars, L.; van der Veer, H.W.; Luttikhuizen, P.C.; Bijleveld, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Klunder, L. <i>et al.</i>","Englishabstract":"DNA metabarcoding methods have been implemented in studies aimed at detecting and quantifying marine benthic biodiversity. In such surveys, universal barcodes are amplified and sequenced from environmental DNA. To quantify biodiversity with DNA metabarcoding, a relation between the number of DNA sequences of a species and its biomass and/or the abundance is required. However, this relationship is complicated by many factors, and it is often unknown. In this study, we validate estimates of biomass and abundance from molecular approaches with those from the traditional morphological approach. Abundance and biomass were quantified from 126 samples of benthic intertidal mudflat using traditional morphological approaches and compared with frequency of occurrence and relative read abundance estimates from a molecular approach. A relationship between biomass and relative read abundance was found for two widely dispersed annelid taxa (<i>Pygospio</i> and <i>Scoloplos</i>). None of the other taxons, however, showed such a relationship. We discuss how quantification of abundance and biomass using molecular approaches are hampered by the ecology of DNA i.e. all the processes that determine the amount of DNA in the environment, including the ecology of the benthic species as well as the compositional nature of sequencing data.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Quantification of marine benthic communities with metabarcoding","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:39.791444","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"abundance; biomass; eDNA; metabarcoding; next- generation sequencing; quantification","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2022-04-08","DateCreate":"2021-11-10","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000713353800001","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/1755-0998.13536"},"refs":null,"anarec":{"AnaID":347291,"PubliDate":2022,"Pagination":"1043-1054","XtraPublOfAnaID":null,"ISBN":null,"Volume":"22","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":124120,"SerRR":"Molecular Ecology Resources. Blackwell Publishing: Oxford.  ISSN 1755-098X; e-ISSN 1755-0998","StandardTitleSer":"Molecular Ecology Resources","ISSN":"1755-098X","AbbrevSer":"Mol. Ecol. Resour.","StandardTitleMon":null,"StartPage":1043,"Pages":12,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Klunder","Firstname":"Lise","Initials":"L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":343983,"AutID":248995,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"COS","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Coastal Sciences","ORCID":null,"PersID":29809,"InsID":13656},{"AutName":"van Bleijswijk","Firstname":"Judith","Initials":"J.D.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":343983,"AutID":242816,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0002-4748-8168","PersID":11075,"InsID":13655},{"AutName":"Kleine Schaars","Firstname":"Loran","Initials":"L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":343983,"AutID":271915,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"COS","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Coastal Sciences","ORCID":null,"PersID":20269,"InsID":13656},{"AutName":"van der Veer","Firstname":"Henk","Initials":"H.W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":343983,"AutID":228982,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"COS","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Coastal Sciences","ORCID":null,"PersID":5044,"InsID":13656},{"AutName":"Luttikhuizen","Firstname":"Pieternella","Initials":"P.C.","Affiliation":"COS","Discriminator":null,"CorporateFlag":0,"BEntID":343983,"AutID":232711,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"COS","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Coastal Sciences","ORCID":null,"PersID":29843,"InsID":13656},{"AutName":"Bijleveld","Firstname":"Allert","Initials":"A.","Affiliation":"COS","Discriminator":null,"CorporateFlag":0,"BEntID":343983,"AutID":289981,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"COS","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Coastal Sciences","ORCID":"0000-0002-3159-8944","PersID":29647,"InsID":13656}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":343983,"BEntPrivate":null,"availability":[{"BInstID":372785,"LibID":36,"BRefID":347291,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"372785","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null},{"BInstID":373087,"LibID":2779,"BRefID":347291,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"373087","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":260,"Classification":"NIOZ Open Repository","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":21,"AccessConstraint":"Attribution (CC BY)","LicURL":"https://creativecommons.org/licenses/by/4.0/"}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Blackwell Publishing","City":"Oxford"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"},"941":{"SpName":"LifeWatch Species Information Backbone","SpColID":941,"ParSpColID":39,"TopParID":39,"ShortName":"LifeWatch Species Information Backbone","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"},"507":{"SpName":"World Register of Marine Species","SpColID":507,"ParSpColID":null,"TopParID":null,"ShortName":"WoRMS website","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"WoRMS website"},"915":{"SpName":"World Register of Marine Species (WoRMS) acknowledged","SpColID":915,"ParSpColID":941,"TopParID":39,"ShortName":"WoRMS ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn"},"947":{"SpName":"WoRMS ackn - direct reference","SpColID":947,"ParSpColID":915,"TopParID":39,"ShortName":"WoRMS ackn - direct","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn/WoRMS ackn - direct"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":343983,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":343983,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1111/1755-0998.13536","externalID":"10.1111/1755-0998.13536","URLTypeCode":"DOI","URLID":97544,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Leonne.van.der.Weegen@nioz.nl","newSesDate":{"date":"2021-11-10 08:38:35.320000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2022-04-08 08:35:35.330000","timezone_type":3,"timezone":"Europe/Brussels"}}}